无需连续稀释即可对HEp-2细胞上的抗核抗体进行精确定量。
Precise quantitation of antinuclear antibodies on HEp-2 cells without the need for serial dilution.
作者信息
Hollingsworth P N, Dawkins R I, Peter J B
机构信息
Royal Perth Hospital, Australia.
出版信息
Clin Diagn Lab Immunol. 1996 Jul;3(4):374-7. doi: 10.1128/cdli.3.4.374-377.1996.
Abstract
Using HEp-2 cells as a substrate, we developed a method to quantitate antinuclear antibodies (ANA) by comparing the green fluorescence intensity of unknown samples with that of calibrated standards. Intensity was then converted to international units per milliliter by reference to a standard curve. This method is accurate and precise around the cutoff for positively (5 to 10 IU/ml) and therefore provides a reliable screening test for active, untreated systemic lupus erythematosus. Furthermore, the method can identify sera likely to contain autoantibodies commonly detected in ANA-positive sera (SS-A, SS-B, Sm, small nuclear ribonucleoprotein, Scl-70, and double-stranded DNA).
摘要
以人喉表皮样癌细胞（HEp-2细胞）为底物，我们开发了一种通过比较未知样品与校准标准品的绿色荧光强度来定量抗核抗体（ANA）的方法。然后通过参考标准曲线将强度转换为每毫升国际单位。该方法在阳性临界值（5至10 IU/ml）附近准确且精密，因此为活动性、未经治疗的系统性红斑狼疮提供了可靠的筛查试验。此外，该方法可以识别可能含有在ANA阳性血清中常见的自身抗体（SS-A、SS-B、Sm、小核糖核蛋白、Scl-70和双链DNA）的血清。
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