疟蚊冈比亚按蚊的物理图谱。
Physical map of the malaria vector Anopheles gambiae.
作者信息
della Torre A, Favia G, Mariotti G, Coluzzi M, Mathiopoulos K D
机构信息
Istituto di Parassitologia, Fondazione Pasteur Cenci-Bolognetti, Università di Roma, La Sapienza, Italy.
出版信息
Genetics. 1996 Jul;143(3):1307-11. doi: 10.1093/genetics/143.3.1307.
Random cDNA clones, cosmid clones and RAPD polymorphic fragments have been localized by in situ hybridization to the ovarian nurse cell polytene chromosomes of the malaria vector Anopheles gambiae. We thus established 85 molecular markers for 110 sites within the whole A. gambiae polytene chromosome complement. The cDNA clones analyzed were isolated at random, and their exact localizations were determined by in situ hybridization. For 15 of the cDNA clones, a partial nucleotide sequence has been obtained; for nine of them sequence searches in the GenBank database revealed high degrees of similarity with published sequences. The cosmid clones analyzed were obtained as the result of screening with a few of the aforementioned cDNA clones of particular interest, or taken from a small set of randomly isolated cosmid clones. The RAPD clones are polymorphic fragments, potentially diagnostic for the various chromosomal forms of A. gambiae that are currently being analyzed.
通过原位杂交，已将随机cDNA克隆、黏粒克隆和RAPD多态性片段定位到疟疾媒介冈比亚按蚊的卵巢滋养细胞多线染色体上。因此，我们在整个冈比亚按蚊多线染色体组的110个位点上建立了85个分子标记。所分析的cDNA克隆是随机分离的，其确切定位通过原位杂交确定。对于15个cDNA克隆，已获得部分核苷酸序列；其中9个在GenBank数据库中的序列搜索显示与已发表序列有高度相似性。所分析的黏粒克隆是用上述一些特别感兴趣的cDNA克隆进行筛选的结果，或者取自一小部分随机分离的黏粒克隆。RAPD克隆是多态性片段，可能对目前正在分析的冈比亚按蚊的各种染色体形式具有诊断作用。
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