Davis W C, MacHugh N D, Park Y H, Hamilton M J, Wyatt C R
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040.
Vet Immunol Immunopathol. 1993 Nov;39(1-3):85-91. doi: 10.1016/0165-2427(93)90167-3.
A monoclonal antibody (mAb), MM1A, that identifies a molecule expressed on a large percentage of bovine lymphocytes (60-80%) was examined to determine its specificity. Two and three colour immunofluorescence analysis using flow cytometry revealed the molecule is highly expressed on all CD4+ and CD8+ lymphocytes and lymphocytes that express WC1 and the gamma/delta TCR. In contrast to mAbs reactive with BoCD5, MM1A did not react with B lymphocytes. Biochemical analysis revealed that the mAb immunoprecipitates a molecular complex comprising a set of peptides with M(r) approximately 12, 16, 22, 32, 36, and 44 kDa under reducing conditions and an additional 96 kDa peptide complex under non-reducing conditions. The data indicate that MM1A recognizes the bovine orthologue of CD3 (BoCD3).
一种识别大量牛淋巴细胞（60 - 80%）上表达分子的单克隆抗体（mAb）MM1A，被用于检测其特异性。使用流式细胞术进行的双色和三色免疫荧光分析显示，该分子在所有CD4 +和CD8 +淋巴细胞以及表达WC1和γ/δTCR的淋巴细胞上高度表达。与与BoCD5反应的单克隆抗体不同，MM1A不与B淋巴细胞反应。生化分析表明，该单克隆抗体在还原条件下免疫沉淀出一个分子复合物，该复合物由一组分子量约为12、16、22、32、36和44 kDa的肽组成，在非还原条件下还沉淀出一个额外的96 kDa肽复合物。数据表明，MM1A识别CD3的牛同源物（BoCD3）。
