人类基因组中的反向重复序列。

Inverted repetitive sequences in the human genome.

作者信息

Dott P J, Chuang C R, Saunders G F

出版信息

Biochemistry. 1976 Sep 7;15(18):4120-5. doi: 10.1021/bi00663a032.

Abstract

A specific class of DNA sequences, the inverted repetitive sequences, forms hairpin-like structures in denatured DNA by the folding back of a single linear chain. The reassociation process of these sequences is unimolecular and the rate is extremely fast. Inverted repetitive sequences comprise 6% of the total human genome. They appear to be heterogeneous in length with an overall average length of 190 nucleotides. The inverted sequences are represented in almost all families of repetition frequencies, highly repetitive as well as very few copies per genome. They are not localized at unique sites on metaphase chromosomes. It is estimated that there are approximately 2 X 10(6) inverted repeats per haploid human genome. The biological function of this class of sequences is unknown.

摘要

一类特定的DNA序列，即反向重复序列，在变性DNA中通过单一线性链的回折形成发夹样结构。这些序列的重新结合过程是单分子的，且速率极快。反向重复序列占人类基因组总量的6%。它们的长度似乎具有异质性，总体平均长度为190个核苷酸。反向序列几乎存在于所有重复频率家族中，包括高度重复的以及每个基因组中拷贝数极少的。它们并不定位于中期染色体上的独特位点。据估计，单倍体人类基因组中大约有2×10⁶个反向重复序列。这类序列的生物学功能尚不清楚。