Yuasa Y, Yoshiie K, Takasaki T, Yoshida H, Oda H
Department of Bacteriology, Kagoshima University, Japan.
J Clin Microbiol. 1996 Apr;34(4):824-7. doi: 10.1128/jcm.34.4.824-827.1996.
We used PCR to detect Coxiella burnetii DNA in paraffin-embedded tissues obtained from patients with chronic endocarditis in which the etiological agent had been unknown. On the basis of the published nucleotide sequence of the C. burnetii htpB gene, primers were chosen to produce an amplified fragment of 285 bp. A total of 60 samples from 56 patients were tested for the presence of C. burnetii DNA. Five samples from four patients were found to be positive. All of the amplified DNA fragments possessed a TthHB8I restriction site, as predicted from the published sequence of C. burnetii. In one of the four positive patients, rickettsia-like particles were found in sections of tissue stained by Gimenez's method. This is the first report of chronic Q fever in Japan.
我们使用聚合酶链反应（PCR）检测从病因不明的慢性心内膜炎患者获取的石蜡包埋组织中的伯氏考克斯体DNA。根据已发表的伯氏考克斯体htpB基因核苷酸序列，选择引物以产生285 bp的扩增片段。对来自56例患者的总共60个样本检测了伯氏考克斯体DNA的存在情况。发现来自4例患者的5个样本呈阳性。所有扩增的DNA片段都具有TthHB8I限制性位点，正如根据已发表的伯氏考克斯体序列所预测的那样。在4例阳性患者中的1例中，通过吉姆尼兹氏法染色的组织切片中发现了立克次体样颗粒。这是日本关于慢性Q热的首次报告。
