阿维链霉菌DSM40697线性染色体DNA上核糖体操纵子的定位
Mapping of the ribosomal operons on the linear chromosomal DNA of Streptomyces ambofaciens DSM40697.
作者信息
Berger F, Fischer G, Kyriacou A, Decaris B, Leblond P
机构信息
Laboratoire de Génétique et Microbiologie, Unité associée INRA 952, Faculté des Sciences, Université Henri Poincaré-Nancy 1, Vandouvre-lès-Nancy, France.
出版信息
FEMS Microbiol Lett. 1996 Oct 1;143(2-3):167-73. doi: 10.1111/j.1574-6968.1996.tb08476.x.
Streptomyces ambofaciens DSM40697 possess six rRNA gene clusters which can be distinguished by probing BfrI-digested total DNA with the PCR-amplified l6S-23S ribosomal genet internal transcribed spacer. The six rrn loci of S. ambofaciens were cloned as recombinant cosmids and located on the AseI-Dŕal physical map of the linear chromosomal DNA. For five of the six ribosomal gene sets, the transcriptional orientation was determined relative to the physical map and was shown to be divergent away from an oriC-like locus.
产二素链霉菌DSM40697拥有六个rRNA基因簇，通过用PCR扩增的16S - 23S核糖体基因内部转录间隔区探测经BfrI酶切的总DNA，可以区分这些基因簇。产二素链霉菌的六个rrn位点被克隆为重组黏粒，并定位在线状染色体DNA的AseI - Dral物理图谱上。对于六个核糖体基因集中的五个，确定了相对于物理图谱的转录方向，结果表明其远离类oriC位点呈发散状。
Zotero 插件