Sibold C, Meisel H, Krüger D H, Labuda M, Lysy J, Kozuch O, Pejcoch M, Vaheri A, Plyusnin A
Institute of Medical Virology, Charité School of Medicine, Humboldt University, D-10098 Berlin, Germany.
J Virol. 1999 Jan;73(1):667-75. doi: 10.1128/JVI.73.1.667-675.1999.
To examine the evolution of Tula hantavirus (TUL), carried by the European common vole (Microtus arvalis and M. rossiaemeridionalis), we have analyzed genetic variants from Slovakia, the country where the virus is endemic. Phylogenetic analysis (PHYLIP) based on either partial (nucleotides [nt] 441 to 898) or complete N-protein-encoding sequences divided Slovakian TUL variants into two main lineages: (i) strains from eastern Slovakia, which clustered with Russian strains, and (ii) strains from western Slovakia situated closer to those from the Czech Republic. We found genetic diversity of 19% between the two groups and 4% within the western Slovakian TUL strains. Phylogenetic analysis of the 3' noncoding region (3'-NCR), however, placed the eastern Slovakian strains closer to those from western Slovakia and the Czech Republic, with a greater distance to the Russian strains, suggesting a recombinant nature of the S segment in the eastern Slovakian TUL lineage. A bootscan search of the S-segment sequences of TUL strains revealed at least two recombination points in the S sequences of eastern Slovakian TUL strains (nt 400 to 415 and around 1200) which agreed well with the pattern of amino acid substitutions in the N protein and deletions/insertions in the 3'-NCR of the S segment. These data suggest that homologous recombination events occurred in the evolution of hantaviruses.
为研究欧洲普通田鼠（草原田鼠和南俄草原田鼠）携带的图拉汉坦病毒（TUL）的进化情况，我们分析了来自该病毒地方性流行国家斯洛伐克的基因变体。基于部分（核苷酸[nt]441至898）或完整的N蛋白编码序列进行的系统发育分析（PHYLIP）将斯洛伐克的TUL变体分为两个主要谱系：（i）来自斯洛伐克东部的毒株，与俄罗斯毒株聚类；（ii）来自斯洛伐克西部的毒株，更接近来自捷克共和国的毒株。我们发现两组之间的遗传多样性为19%，斯洛伐克西部的TUL毒株内部为4%。然而，对3'非编码区（3'-NCR）的系统发育分析表明，斯洛伐克东部的毒株更接近斯洛伐克西部和捷克共和国的毒株，与俄罗斯毒株的距离更远，这表明斯洛伐克东部TUL谱系中S片段具有重组性质。对TUL毒株S片段序列的bootscan搜索显示，斯洛伐克东部TUL毒株的S序列中至少有两个重组点（nt 400至415和1200左右），这与N蛋白中的氨基酸取代模式以及S片段3'-NCR中的缺失/插入情况非常吻合。这些数据表明，汉坦病毒进化过程中发生了同源重组事件。
