Zaoming W, Codina R, Fernández-Caldas E, Lockey R F
Department of Allergy, Peking Union Medical College, Beijing, China.
J Investig Allergol Clin Immunol. 1996 Jul-Aug;6(4):237-41.
Although there have been reports of allergic reactions such as asthma and hypersensitivity pneumonitis to silk allergens (especially in oriental countries), these allergens have not been characterized. We report an in vivo and in vitro study of subjects allergic to silk, as well as a partial characterization of the allergens involved. Forty-one subjects with a clinical history of silk allergy who had asthma, allergic rhinitis or both, and who had a positive intradermal skin test to mulberry silk extract, were evaluated in the study. Four asthmatic subjects with negative skin tests and RASTs to mulberry silk extract were used as controls. RAST specific lgE was present in 37/41 (90%) subjects. SDS-PAGE of mulberry silk extract demonstrated 13 protein bands of less than 21-95 kD. lgE which bound to protein bands was present in 13/32 (41%) sera, twelve of which bound to a single protein with a molecular weight between 35.1 and 50 kD. A pool made with the four control sera did not react with any protein band. The patients allergic to silk showed evidence of sensitivity to mulberry silk extract, both in vivo and in vitro, but sera from 19/32 (59%) subjects did not have antibodies reactive with the mulberry silk extract immunoblot protein bands. The low avidity of the lgE may be responsible for this discrepancy between in vivo and in vitro results.
尽管已有关于对丝绸过敏原（尤其是在东方国家）出现过敏反应如哮喘和过敏性肺炎的报道，但这些过敏原尚未得到鉴定。我们报告了一项针对对丝绸过敏的受试者的体内和体外研究，以及对相关过敏原的部分鉴定。本研究评估了41名有丝绸过敏临床病史、患有哮喘、过敏性鼻炎或两者皆有，且对桑蚕丝提取物皮内皮肤试验呈阳性的受试者。4名对桑蚕丝提取物皮肤试验和放射性过敏原吸附试验（RAST）均为阴性的哮喘受试者用作对照。37/41（90%）的受试者存在RAST特异性IgE。桑蚕丝提取物的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）显示出13条分子量小于21 - 95 kD的蛋白条带。与蛋白条带结合的IgE存在于13/32（41%）的血清中，其中12份血清与一条分子量在35.1至50 kD之间的单一蛋白结合。由4份对照血清制成的混合血清与任何蛋白条带均无反应。对丝绸过敏的患者在体内和体外均显示出对桑蚕丝提取物敏感的证据，但19/32（59%）受试者的血清没有与桑蚕丝提取物免疫印迹蛋白条带发生反应的抗体。IgE的低亲和力可能是体内和体外结果之间存在这种差异的原因。
