Diamond R D
Infect Immun. 1977 Jul;17(1):187-94. doi: 10.1128/iai.17.1.187-194.1977.
A model of cryptococcosis was developed using intraperitoneal infections of guinea pigs. This model shared characteristics with cryptococcosis in humans and was used to study the effects of immunosuppression and immunostimulation on cryptococcosis. Female guniea pigs survived longer than males; perphaps this was related to a greater capacity of their monocytes to kill cryptococci. A brief course of cortisone shortened survival of females and resulted in depressed immune and inflammatory responses, which persisted long after cortisone was stopped. Stimulation of the immune response by treatment with cryptococci in Freund complete adjuvant improved survival in males. Preliminary studies indicated the usefulness of this model for the study of other potential immunostimulants, including immune lymphocytes, transfer factor prepared from immune lymphocytes, and levamisole. Overall, long-term survivors appeared to clear disseminated cryptococci from extraperitoneal sites including brain, rather than prevent dissemination of cryptococci from the peritoneal cavity. The quantity of the inflammatory response in infectious foci, rather than the ability of individual leukocytes to kill crytococci, may have determined the outcome of most infections.
通过对豚鼠进行腹腔感染建立了隐球菌病模型。该模型具有人类隐球菌病的特征，用于研究免疫抑制和免疫刺激对隐球菌病的影响。雌性豚鼠比雄性存活时间更长；这可能与其单核细胞杀灭隐球菌的能力更强有关。短期使用可的松会缩短雌性豚鼠的存活时间，并导致免疫和炎症反应受到抑制，这种抑制在停止使用可的松后仍会持续很长时间。用弗氏完全佐剂中的隐球菌进行治疗来刺激免疫反应可提高雄性豚鼠的存活率。初步研究表明该模型对于研究其他潜在的免疫刺激剂有用，这些免疫刺激剂包括免疫淋巴细胞、从免疫淋巴细胞制备的转移因子以及左旋咪唑。总体而言，长期存活者似乎清除了包括脑在内的腹膜外部位的播散性隐球菌，而不是阻止隐球菌从腹腔播散。感染病灶中炎症反应的程度，而非单个白细胞杀灭隐球菌的能力，可能决定了大多数感染的结果。
