Mitchell J B, Paquet A J, Pizza F X, Starling R D, Holtz R W, Grandjean P W
Department of Physical Education, Texas Christian University, Ft. Worth, USA.
Int J Sports Med. 1996 Jul;17(5):384-9. doi: 10.1055/s-2007-972865.
The purpose of this study was to determine the effect of 12 wks of aerobic training on resting lymphocyte number and proliferation, and immunoglobulin and cytokine levels. Eleven college-aged males (training group = EX) performed 30 min of cycling at 75% of VO2peak, 3 days/wk with VO2peak assessment and blood samples taken at 0,8 and 12 wks. A group of 10 sedentary controls (CT) underwent the same testing protocol. Lymphocyte proliferation response to phytohemagglutinin (PHA) and pokeweed mitogen (PWM) was quantified as a stimulation index (SI) based on the ratio of stimulated versus control cultures, and as total counts per min (CPM). Immunoglobulin (Ig) levels (IgG, IgA, and IgM), and lymphocyte counts were also determined. There was a significant increase in VO2 in the EX group (41.0 +/- 1.8 vs. 46.3 +/- 1.4 ml.kg-1.min-1 pre and post training, respectively). Training had no effect on the PHA SI for the EX group (23.9 +/- 3.3, 27.7 +/- 4.1, and 26.3 +/- 4.0 at 0, 8 and 12 wks, respectively), or the responses of the CT group (28.8 +/- 6.0, 23.9 +/- 3.1, and 30.6 +/- 4.3). No changes were observed for the PWM SI. Significant increases were observed in the CPM for both groups. No differences in the Ig or lymphocyte levels were found during the study. These data indicate that 12 wks of moderate endurance training did not alter resting immune function as determined by mitogen stimulated lymphocyte proliferation, total circulating lymphocytes, or Ig levels.
本研究的目的是确定12周有氧训练对静息淋巴细胞数量与增殖、免疫球蛋白及细胞因子水平的影响。11名大学年龄男性（训练组=EX）以VO2峰值的75%进行30分钟的骑行，每周3天，在第0、8和12周进行VO2峰值评估并采集血样。一组10名久坐不动的对照者（CT）接受相同的测试方案。根据刺激培养物与对照培养物的比率，将淋巴细胞对植物血凝素（PHA）和商陆有丝分裂原（PWM）的增殖反应量化为刺激指数（SI），并以每分钟总计数（CPM）表示。还测定了免疫球蛋白（Ig）水平（IgG、IgA和IgM）以及淋巴细胞计数。EX组的VO2有显著增加（训练前和训练后分别为41.0±1.8与46.3±1.4 ml·kg-1·min-1）。训练对EX组的PHA SI无影响（在第0、8和12周分别为23.9±3.3、27.7±4.1和26.3±4.0），对CT组的反应也无影响（分别为28.8±6.0、23.9±3.1和30.6±4.3）。未观察到PWM SI有变化。两组的CPM均有显著增加。在研究期间未发现Ig或淋巴细胞水平有差异。这些数据表明，12周的中等耐力训练并未改变由有丝分裂原刺激的淋巴细胞增殖、循环淋巴细胞总数或Ig水平所确定的静息免疫功能。
