Damgaard P H, Larsen H D, Hansen B M, Bresciani J, Jørgensen K
Department of Ecology and Molecular Biology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Lett Appl Microbiol. 1996 Sep;23(3):146-50. doi: 10.1111/j.1472-765x.1996.tb00051.x.
Strains of Bacillus thuringiensis were isolated from various food items (pasta, pitta bread and milk) and were found to belong to either H-serotype kurstaki or neoleonensis. The strains were bioassayed against Pieris brassicae and insecticidal activity of strains was found to correspond to the presence of the cry1A-gene. All strains, except one, were found to express cytotoxic effects on Vero cells as an indicator of enterotoxin activity. Further, the B. thuringiensis strains HD-1 (serotype kurstaki), NB-125 (serotype tenebrionis) and HD-567 (serotype israelensis ) which are used commercially for insect pest management, were also found to have cytotoxic effects on Vero cells.
苏云金芽孢杆菌菌株是从各种食品（意大利面、皮塔饼和牛奶）中分离出来的，发现它们属于库尔斯塔克H血清型或新莱昂血清型。对这些菌株进行了针对粉纹夜蛾的生物测定，发现菌株的杀虫活性与cry1A基因的存在相对应。除了一株菌株外，所有菌株都对Vero细胞表现出细胞毒性作用，以此作为肠毒素活性的指标。此外，商业上用于害虫管理的苏云金芽孢杆菌菌株HD-1（库尔斯塔克血清型）、NB-125（黄粉虫血清型）和HD-567（以色列血清型）也被发现对Vero细胞有细胞毒性作用。
