Imberechts H, Wild P, Charlier G, De Greve H, Lintermans P, Pohl P
Laboratory of General Bacteriology, National Veterinary Research Institute, Brussels, Belgium.
Microb Pathog. 1996 Sep;21(3):183-92. doi: 10.1006/mpat.1996.0053.
Infection of susceptible weaned pigs with oedema disease strains of E. coli is associated with bacterial adhesion to the small intestine. F18 fimbria (previously named F107) was the first colonisation factor described on oedema disease strains, and its genetic determinant was cloned. In the present study, genes fedE and fedF were positioned in the F18 gene cluster, downstream of the major structural subunit gene fedA. Two fedE and two fedF mutants were identified that had lost their capacity to adhere to isolated porcine villi. Moreover, these mutants produced significantly longer fimbriae. In vitro adhesion tests, electron microscopy study, transcomplementation tests, and nucleotide sequence analysis indicated that proteins FedE and FedF are F18 minor subunits essential for fimbrial adhesion and effecting fimbrial length.
易感断奶仔猪感染大肠杆菌水肿病菌株与细菌粘附于小肠有关。F18菌毛（以前称为F107）是在水肿病菌株上描述的第一个定植因子，其遗传决定簇已被克隆。在本研究中，fedE和fedF基因位于F18基因簇中，在主要结构亚基基因fedA的下游。鉴定出两个fedE突变体和两个fedF突变体，它们失去了粘附分离的猪绒毛的能力。此外，这些突变体产生的菌毛明显更长。体外粘附试验、电子显微镜研究、互补试验和核苷酸序列分析表明，FedE和FedF蛋白是菌毛粘附和影响菌毛长度所必需的F18次要亚基。
