Väisänen S, Rauramaa R, Rankinen T, Gagnon J, Couchard C
Kuopio Research Institute of Exercise Medicine, University of Kuopio, Finland.
Med Sci Sports Exerc. 1996 Sep;28(9):1165-70. doi: 10.1097/00005768-199609000-00013.
The relation of daily energy expenditure (EE) and maximal oxygen uptake (VO2max) to plasma fibrinogen with reference to DNA polymorphism was analyzed in a random sample of men (N = 189), age 50-60. Fibrinogen polymorphism was detected using polymerase chain reaction (PCR) and digestion with HindIII and BclI (beta-fibrinogen), and TaqI (alpha-fibrinogen) restriction enzymes. Mean VO2max was 29.4 ml.kg-1.min-1 (95%CI 28.5; 30.2) and mean daily EE was 179 kJ.kg-1.d-1 (173; 186) and were similar in all fibrinogen genotypes. Plasma fibrinogen was 3.26 g.1-1 (3.18; 3.34) and did not associate with fibrinogen polymorphisms. Both EE and VO2max related inversely to fibrinogen level (r = -0.24, P < 0.001). Strongest predictors of plasma fibrinogen were VO2max in TaqI 800 bp homozygotes, and EE together with smoking in TaqI 900 bp homozygotes. The predictive role of VO2max was marginal in the common beta-fibrinogen genotypes, whereas physical activity level explained up to 9% of the variance in the less frequent genotypes. These data suggest that the association of VO2max and EE with plasma fibrinogen varies across the fibrinogen genotypes.
在一个年龄为50 - 60岁的男性随机样本（N = 189）中，分析了每日能量消耗（EE）和最大摄氧量（VO2max）与血浆纤维蛋白原之间的关系，并参考了DNA多态性。使用聚合酶链反应（PCR）以及用HindIII和BclI（β - 纤维蛋白原）和TaqI（α - 纤维蛋白原）限制性内切酶进行消化来检测纤维蛋白原多态性。平均VO2max为29.4 ml·kg-1·min-1（95%CI 28.5；30.2），平均每日EE为179 kJ·kg-1·d-1（173；186），在所有纤维蛋白原基因型中相似。血浆纤维蛋白原为3.26 g·1-1（3.18；3.34），与纤维蛋白原多态性无关。EE和VO2max均与纤维蛋白原水平呈负相关（r = -0.24，P < 0.001）。血浆纤维蛋白原的最强预测因素在TaqI 800 bp纯合子中是VO2max，在TaqI 900 bp纯合子中是EE与吸烟。VO2max在常见的β - 纤维蛋白原基因型中的预测作用很小，而身体活动水平在较罕见的基因型中解释了高达9%的变异。这些数据表明，VO2max和EE与血浆纤维蛋白原之间的关联在纤维蛋白原基因型中有所不同。
