在改良的Myro液体培养基中，串珠镰刀菌M5991菌株产生伏马毒素B1的情况。

Fumonisin B1 production by Fusarium proliferatum strain M5991 in a modified Myro liquid medium.

作者信息

Dantzer W R, Pometto A L, Murphy P A

机构信息

Department of Food Science and Human Nutrition, Iowa State University, Ames 50011, USA.

出版信息

Nat Toxins. 1996;4(4):168-73. doi: 10.1002/19960404nt4.

DOI:10.1002/19960404nt4

PMID:8887947

Abstract

Fusarium proliferatum strain M5991 cultures were grown in shake flasks containing modified Myro (MM) medium (MgSO4 reduced to 0.5 g/L) plus 0, 0.25, 0.50, 0.75, 1.00, or 1.25% (v/v) hot-water and corn-hull-extract (CHE) for 69 days. After 4 days of incubation, shake flask liquid cultures with 0.75, 1.00, and 1.25% (v/v) CHE showed a reduction in pH from 6.0 to 2.6 and consumed sucrose at > 6.3 g/L/d. After 69 days of incubation, the same shake flask cultures produced over 7.8 g/L cell mass and over 990 mg/L fumonisin B1 (FB1). A minimum CHE level of 0.75% was recommended for enhanced FB1 production by F. proliferatum strain M5991. During three serial (10, 12, and 12 L) batch fermentations in MM medium + 1.00% (v/v) CHE (first batch only), F. proliferatum strain M5991 produced FB1 concentrations of 619, 659, and 375 mg/L after 35, 47, and 52 days of incubation, respectively. By analysis, a total yield of 20 g FB1 was obtained from three serial batch fermentations.

摘要

将层出镰刀菌菌株M5991接种于装有改良迈罗（MM）培养基（硫酸镁含量降至0.5 g/L）并添加0、0.25%、0.50%、0.75%、1.00%或1.25%（v/v）热水和玉米皮提取物（CHE）的摇瓶中培养69天。培养4天后，含有0.75%、1.00%和1.25%（v/v）CHE的摇瓶液体培养物的pH从6.0降至2.6，蔗糖消耗速率>6.3 g/L/天。培养69天后，相同的摇瓶培养物产生了超过7.8 g/L的细胞量和超过990 mg/L的伏马毒素B1（FB1）。建议CHE的最低添加水平为0.75%，以提高层出镰刀菌菌株M5991的FB1产量。在MM培养基+1.00%（v/v）CHE（仅第一批）中进行的三次连续（10 L、12 L和12 L）分批发酵过程中，层出镰刀菌菌株M5991在培养35天、47天和52天后分别产生了浓度为619 mg/L、659 mg/L和375 mg/L的FB1。经分析，三次连续分批发酵共获得20 g FB1。