Brenner I K, Severs Y D, Shek P N, Shephard R J
Graduate Department of Community Health, University of Toronto, Ontario, Canada.
Eur J Appl Physiol Occup Physiol. 1996;74(1-2):162-71. doi: 10.1007/BF00376509.
This study examined the impact of heat exposure and moderate, intermittent exercise on the CD16+ and CD56+ cell counts and cytolytic activity. Eleven healthy male subjects [mean (SD): age = 27.1 (3.0) years, peak oxygen intake, VO2peak = 47.6 (6.2) ml. kg-1. min-1] were assigned to each of four different experimental conditions according to a randomized-block design. While in a climatic chamber maintained at a comfortable temperature (23 degrees C) or heated (40 degrees C, 30% relative humidity, r.h.), subjects performed either two 30-min bouts of cycle-ergometer exercise at approximately 50% VO2peak (separated by a 45-min recovery interval), or remained seated for 3 h. Blood samples were analyzed for CD16+ and CD56+ cell counts, cytolytic activity and the concentrations of various exercise stress hormones (norepinephrine, epinephrine and cortisol). Heat exposure alone had no significant effect on cytolytic cells. The (CD16+ and CD56+) cell count increased significantly (P < 0.0001) during each exercise bout under both environmental conditions, but returned to baseline levels 15-45 min following each exercise bout. Total cytolytic activity (determined by a standard 51Cr release assay using K562 cells) followed a similar pattern, but cytolytic activity per CD16+ or CD56+ cell was not significantly modified by exercise. Our findings show a strong association between hemodynamic factors and recruitment of cytolytic cells into the peripheral circulation. Alterations in cytolytic activity of the whole blood during and following moderate exercise seem to be the result of changes in CD16+ and CD56+ cell counts.
本研究考察了热暴露以及适度的间歇性运动对CD16 +和CD56 +细胞计数及细胞溶解活性的影响。根据随机区组设计，将11名健康男性受试者[平均（标准差）：年龄 = 27.1（3.0）岁，峰值摄氧量，VO2peak = 47.6（6.2）ml·kg-1·min-1]分配到四种不同的实验条件中。受试者在保持舒适温度（23摄氏度）或加热（40摄氏度，相对湿度30%，r.h.）的气候舱内，要么以约50%VO2peak进行两轮30分钟的自行车测力计运动（中间间隔45分钟恢复时间），要么静坐3小时。对血样进行分析，检测CD16 +和CD56 +细胞计数、细胞溶解活性以及各种运动应激激素（去甲肾上腺素、肾上腺素和皮质醇）的浓度。单独热暴露对细胞溶解细胞无显著影响。在两种环境条件下，每次运动期间（CD16 +和CD56 +）细胞计数均显著增加（P < 0.0001），但每次运动后15 - 45分钟恢复至基线水平。总细胞溶解活性（通过使用K562细胞的标准51Cr释放试验测定）呈现类似模式，但运动并未显著改变每个CD16 +或CD56 +细胞的细胞溶解活性。我们的研究结果表明血流动力学因素与细胞溶解细胞募集到外周循环之间存在密切关联。适度运动期间及之后全血细胞溶解活性的改变似乎是CD16 +和CD56 +细胞计数变化的结果。
