Groeneveld P H, Kroon F P, Nibbering P H, Bruisten S M, van Swieten P, van Furth R
Department of Infectious Diseases, University Hospital Leiden, The Netherlands.
Scand J Infect Dis. 1996;28(4):341-5. doi: 10.3109/00365549609037916.
The objective of our study was to determine the production of nitric oxide (NO) in patients infected with human immunodeficiency virus (HIV) and its relation to cellular immunity, activation of mononuclear phagocytes and the amount of circulating virus. Therefore, serum nitrate, the stable metabolite of NO, the number of peripheral CD4+ T-lmphocytes, serum neopterin, plasma HIV-RNA and HIV-DNA in peripheral blood mononuclear cells of afebrile HIV-infected patients were determined. Serum nitrate levels were significantly (p = 0.002) increased in HIV-infected patients (median 37 microM, range 13-137 microM, n = 77) in comparison to healthy subjects (median 28 microM, range 21-40 microM, n = 17). Serum nitrate levels did not correlate with the number of CD4+ T-lymphocytes (r = 0.05, p > 0.05). Serum nitrate levels were positively correlated with neopterin (r = 0.36, p = 0.05, n = 30), the amount of HIV-DNA in peripheral blood mononuclear cells (r = 0.63, p < 0.001, n =27) and plasma HIV-RNA levels (r = 0.35, p = 0.08, n = 27). A possible explanation of our findings is that HIV induces the production of NO by means of activated mononuclear phagocytes.
我们研究的目的是确定感染人类免疫缺陷病毒（HIV）患者体内一氧化氮（NO）的产生情况及其与细胞免疫、单核吞噬细胞活化和循环病毒量的关系。因此，我们测定了无发热症状的HIV感染患者血清中NO的稳定代谢产物硝酸盐水平、外周血CD4⁺ T淋巴细胞数量、血清新蝶呤、外周血单核细胞中的血浆HIV-RNA和HIV-DNA。与健康受试者（中位数28μM，范围21 - 40μM，n = 17）相比，HIV感染患者的血清硝酸盐水平显著升高（p = 0.002）（中位数37μM，范围13 - 137μM，n = 77）。血清硝酸盐水平与CD4⁺ T淋巴细胞数量无相关性（r = 0.05，p > 0.05）。血清硝酸盐水平与新蝶呤呈正相关（r = 0.36，p = 0.05，n = 30），与外周血单核细胞中HIV-DNA的量呈正相关（r = 0.63，p < 0.001，n = 27），与血浆HIV-RNA水平呈正相关（r = 0.35，p = 0.08，n = 27）。对我们研究结果的一种可能解释是，HIV通过活化的单核吞噬细胞诱导NO的产生。
