Doi H, Apichartpiyakul C, Ohba K I, Mizokami M, Hotta H
Department of Microbiology, Kobe University School of Medicine, Japan.
J Clin Microbiol. 1996 Mar;34(3):569-74. doi: 10.1128/jcm.34.3.569-574.1996.
Subtype analysis of hepatitis C viruses (HCVs) obtained from patients with chronic liver disease in Chiang Mai, Thailand, was performed. Of 46 HCV isolates, 13 (28%) were shown to belong to HCV subtype 3a (HCV-3a), 10 (22%) to belong to HCV-1a, 7 (15%) to belong to HCV-1b, 1 (2%) to belong to HCV-3b, and 1 (2%) to belong to a variant group, as determined from partial nucleotide sequences of the NS5B region of the viral genome. Analysis of 5' untranslated region sequences identified five other isolates (11%) of HCV type 1 and two other isolates (4%) of type 3. Detailed phylogenetic positions for the variant described above and those previously obtained from blood donors and drug addicts in Chiang Mai were determined by a six-parameter neighbor-joining method on the basis of core, E1, and NS5B region sequences. The results revealed that those sequence variants represent novel subtypes of HCV type 6. The HCV type 6 isolates appear to be antigenically different from isolates of HCV types 1 and 2, as determined by a serotyping method that utilizes recombinant peptides corresponding to a portion of the NS4 protein. The significance of subtype analysis around this area is discussed.
对从泰国清迈慢性肝病患者身上获取的丙型肝炎病毒（HCV）进行了亚型分析。在46株HCV分离株中，根据病毒基因组NS5B区域的部分核苷酸序列确定，13株（28%）属于HCV 3a亚型（HCV-3a），10株（22%）属于HCV-1a，7株（15%）属于HCV-1b，1株（2%）属于HCV-3b，1株（2%）属于一个变异组。对5'非翻译区序列的分析鉴定出另外5株（11%）HCV 1型分离株和另外2株（4%）HCV 3型分离株。根据核心区、E1区和NS5B区序列，通过六参数邻接法确定了上述变异株以及先前从清迈献血者和吸毒者身上获得的变异株的详细系统发育位置。结果显示，这些序列变异代表了HCV 6型的新型亚型。通过利用对应于NS4蛋白一部分的重组肽的血清分型方法确定，HCV 6型分离株在抗原性上似乎与HCV 1型和2型分离株不同。本文讨论了该地区亚型分析的意义。
