Yokoyama M, Natsuizaka T, Ishii Y, Ohshima S, Kasagi A, Tateno S
Cancer. 1977 Aug;40(2):766-72. doi: 10.1002/1097-0142(197708)40:2<766::aid-cncr2820400225>3.0.co;2-d.
A case of lung cancer with elevated amylase activity in serum, urine, and tumor tissue was studied electron microscopically and biochemically. Ultrastructurally, there were numerous electron-dense granules in the cytoplasm of most tumor cells. These granules were located in the apical region of tumor cells and had a single limiting membrane, associated with a clear zone just beneath it. Furthermore, circular, lamellar and annulate structures, which closely resembled those observed within zymogen granules of the salivary glands in postnatal mice and rats, were sometimes recognized within these granules. These observations suggested that these granules were identical to a postnatal or immature form of zymogen granules in salivary glands. Biochemical analysis confirmed that the amylases obtained from the tumor tissue were composed of the sialic acid-containing glycoprotein, which was not found in normal amylases. A possible histogenesis of the amylase-producing lung cancer was briefly discussed.
对一例血清、尿液和肿瘤组织中淀粉酶活性升高的肺癌患者进行了电子显微镜和生化研究。在超微结构上，大多数肿瘤细胞的细胞质中有大量电子致密颗粒。这些颗粒位于肿瘤细胞的顶端区域，有一层单层界膜，其下方有一个透明区。此外，在这些颗粒中有时可识别出圆形、层状和环状结构，它们与出生后小鼠和大鼠唾液腺的酶原颗粒中观察到的结构非常相似。这些观察结果表明，这些颗粒与唾液腺中出生后或未成熟形式的酶原颗粒相同。生化分析证实，从肿瘤组织中获得的淀粉酶由含唾液酸的糖蛋白组成，这在正常淀粉酶中未发现。本文简要讨论了产生淀粉酶的肺癌可能的组织发生。
