Stranahan P L, Laroe J, McCombs R, Goldsmith A, Rahim I, Overland M, Pettijohn D E
University of Colorado Cancer Center, Denver 80262, USA.
Glycoconj J. 1996 Oct;13(5):741-7. doi: 10.1007/BF00702338.
Intratumoral phenotypic diversity is well documented with regard to tumor associated carbohydrate antigens (TACA). The factors which control the expression of these cell-surface oligosaccharides on different cells of the same tumor are not understood. We investigated the expression of a panel of mucin associated oligosaccharides in cell lines growing at different surface densities (number of cells per cm2 of growth flask). Results show that the apparent expression of extended Lea-Lex, Lea and Lex, sialyl Lea, Tn and sialyl Tn varies with density of growth by an invasive human squamous cell lung carcinoma cell line (NU6-1), a benign variant (NE-18) and the human lung epithelial cell line BEAS-2B. The results indicate that one of the factors influencing the apparent expression of mucin-associated oligosaccharides is cell-cell interactions.
肿瘤内表型多样性在肿瘤相关碳水化合物抗原（TACA）方面已有充分记录。但控制同一肿瘤不同细胞上这些细胞表面寡糖表达的因素尚不清楚。我们研究了一组与粘蛋白相关的寡糖在以不同表面密度（每平方厘米生长瓶的细胞数）生长的细胞系中的表达。结果表明，延伸型Lea-Lex、Lea和Lex、唾液酸化Lea、Tn和唾液酸化Tn的表观表达随侵袭性人肺鳞状细胞癌细胞系（NU6-1）、良性变体（NE-18）和人肺上皮细胞系BEAS-2B的生长密度而变化。结果表明，影响粘蛋白相关寡糖表观表达的因素之一是细胞间相互作用。
