Cui S, Hano H, Sakata A, Harada T, Liu T, Takai S, Ushigome S
Department of Pathology, Jikei University School of Medicine, Tokyo, Japan.
Pathol Int. 1996 Oct;46(10):751-6. doi: 10.1111/j.1440-1827.1996.tb03544.x.
The immunohistochemical expression of CD34 (human hematopoietic stem cell and endothelial cell marker) and laminin were studied in chronic liver diseases and hepatocellular carcinoma (HCC) to elucidate whether their expression reflected phenotypic differences between non-cancerous sinusoids and sinusoid-like tumor vessels. In normal liver, hepatic sinusoids were always negative for CD34 and laminin. In chronic hepatitis and cirrhosis, the two antigens were sparsely expressed in capillarized sinusoids at periportal and perinodular area. In advanced HCC, CD34 was strongly and diffusely expressed by the endothelial lining of sinusoid-like tumor vessels. However, early-stage HCC showed a wide spectrum of CD34 expression from negative to focal and diffuse, strongly positive staining in sinusoid-like vessels. Laminin was strongly expressed in advanced HCC but not in early-stage HCC. The results indicate that the enhanced expression of CD34 by sinusoidal endothelial cells may reflect the phenotypic change of endothelial cells in chronic liver diseases and HCC, and that the expression may correlate with the processes of angiogenesis induced by hepatocarcinogenesis.
研究了慢性肝病和肝细胞癌（HCC）中CD34（人类造血干细胞和内皮细胞标志物）和层粘连蛋白的免疫组化表达，以阐明它们的表达是否反映了非癌性肝血窦和类肝血窦肿瘤血管之间的表型差异。在正常肝脏中，肝血窦的CD34和层粘连蛋白始终呈阴性。在慢性肝炎和肝硬化中，这两种抗原在门周和结节周围区域的毛细血管化肝血窦中稀疏表达。在晚期HCC中，类肝血窦肿瘤血管的内皮衬里强烈且弥漫性表达CD34。然而，早期HCC显示出从阴性到局灶性和弥漫性的广泛CD34表达，在类肝血窦血管中呈强阳性染色。层粘连蛋白在晚期HCC中强烈表达，但在早期HCC中不表达。结果表明，肝血窦内皮细胞CD34表达增强可能反映了慢性肝病和HCC中内皮细胞的表型变化，且该表达可能与肝癌发生诱导的血管生成过程相关。
