Kobayashi Y, Horimoto T, Kawaoka Y, Alexander D J, Itakura C
Laboratory of Comparative Pathology, Hokkaido University, Sapporo, Japan.
J Comp Pathol. 1996 Feb;114(2):131-47. doi: 10.1016/s0021-9975(96)80003-x.
Central nervous system lesions of chickens inoculated with three highly pathogenic avian influenza virus strains, A/chicken/Victoria/1/85 (H7N7), A/turkey/England/50-92/91 (H5N1), and A/tern/South Africa/61 (H5N3), were examined histologically and immunohistochemically. The chickens either died within 7 days of inoculation or were killed 2 weeks after inoculation. No significant differences were observed in the lesions induced by these three viruses. The lesions were divided into two types, disseminated foci of microgliosis and necrosis, and ventriculitis. The former lesions were associated with infection of the vascular endothelium and dissemination of the virus to the peripheral parenchymal cells of the chickens that died within 3 days of inoculation. The ventriculitis lesions, however, were observed mainly in the chickens that died between 4 and 7 days after inoculation. These findings suggest that viral infection of the vascular endothelium and subsequent involvement of ependymal cells play important roles in the pathogenesis of the central nervous system lesions.
对接种三种高致病性禽流感病毒毒株（A/鸡/维多利亚/1/85（H7N7）、A/火鸡/英格兰/50 - 92/91（H5N1）和A/燕鸥/南非/61（H5N3））的鸡的中枢神经系统病变进行了组织学和免疫组织化学检查。这些鸡要么在接种后7天内死亡，要么在接种后2周被处死。在这三种病毒引起的病变中未观察到显著差异。病变分为两种类型，即弥漫性小胶质细胞增生和坏死灶以及脑室炎。前一种病变与血管内皮感染以及病毒传播到接种后3天内死亡的鸡的外周实质细胞有关。然而，脑室炎病变主要在接种后4至7天死亡的鸡中观察到。这些发现表明，血管内皮的病毒感染以及随后室管膜细胞的受累在中枢神经系统病变的发病机制中起重要作用。
