Bayer M E, Zhang L, Bayer M H
Fox Chase Cancer Center, Philadelphia, PA 19111, USA.
Infection. 1996 Sep-Oct;24(5):347-53. doi: 10.1007/BF01716077.
The presence of Borrelia burgdorferi DNA was established by PCR from urine samples of 97 patients clinically diagnosed as presenting with symptoms of chronic Lyme disease. All patients had shown erythema chronica migrans following a deer tick bite. Most of the patients had been antibiotic-treated for extended periods of time. We used three sets of primer pairs with DNA sequences for the gene coding of outer surface protein A (OspA) and of a genomic sequence of B. burgdorferi to study samples of physician-referred patients from the mideastern USA. Controls from 62 healthy volunteers of the same geographic areas were routinely carried through the procedures in parallel with patients' samples. Of the 97 patients, 72 (74.2%) were found with positive PCR and the rest with negative PCR. The 62 healthy volunteers were PCR negative. It is proposed that a sizeable group of patients diagnosed on clinical grounds as having chronic Lyme disease may still excrete Borrelia DNA, and may do so in spite of intensive antibiotic treatment.
通过聚合酶链反应（PCR）从97例临床诊断为患有慢性莱姆病症状的患者尿液样本中确定了伯氏疏螺旋体DNA的存在。所有患者在被鹿蜱叮咬后均出现慢性游走性红斑。大多数患者接受了长时间的抗生素治疗。我们使用了三组引物对，其DNA序列用于编码外表面蛋白A（OspA）的基因以及伯氏疏螺旋体的基因组序列，以研究来自美国中西部地区由医生转诊患者的样本。来自同一地理区域的62名健康志愿者的对照样本与患者样本并行常规进行检测。在97例患者中，72例（74.2%）PCR检测呈阳性，其余患者PCR检测呈阴性。62名健康志愿者的PCR检测为阴性。有人提出，相当一部分临床上被诊断为患有慢性莱姆病的患者可能仍会排出伯氏疏螺旋体DNA，并且尽管接受了强化抗生素治疗仍可能如此。
