Eiffert H, Karsten A, Thomssen R, Christen H J
Department of Medical Microbiology, Georg-August-University Göttingen, Germany.
Scand J Infect Dis. 1998;30(3):265-8. doi: 10.1080/00365549850160918.
In the study presented, we investigated whether Lyme arthritis is associated with a particular Borrelia burgdorferi genospecies. Using the PCR technique, in 7/11 samples of synovial fluid of patients with Lyme arthritis a part of the ospA-gene was identified and the strains characterized by sequencing of the amplified DNA. Borrelia burgdorferi sensu stricto was found in 3 patients, B. garinii in 3, and B. afzelii in 1 patient. In conclusion, Lyme arthritis is caused by all 3 human pathogenetic genospecies which are actually known. For clinical practice PCR proved to be a rather insensitive diagnostic method, but may confirm the diagnosis of Lyme arthritis in doubtful cases.
在本研究中，我们调查了莱姆关节炎是否与特定的伯氏疏螺旋体基因种相关。使用聚合酶链反应（PCR）技术，在11例莱姆关节炎患者的滑膜液样本中，有7例鉴定出了ospA基因的一部分，并通过对扩增DNA进行测序来鉴定菌株。在3例患者中发现了狭义伯氏疏螺旋体，3例中发现了伽氏疏螺旋体，1例中发现了阿氏疏螺旋体。总之，莱姆关节炎是由目前已知的所有3种人类致病基因种引起的。对于临床实践而言，PCR被证明是一种相当不敏感的诊断方法，但在可疑病例中可用于确诊莱姆关节炎。
