Traoré O, Fayard S F, Laveran H
Service d'hygiène hospitalière, Faculté de Médecine, Clermont Ferrand, France.
J Hosp Infect. 1996 Nov;34(3):217-22. doi: 10.1016/s0195-6701(96)90069-9.
Two povidone-iodine (PVP-I) preparations, one, an antiseptic handwash and one, a skin disinfectant, were tested against 504 bacterial strains isolated from nosocomial infections in 12 French hospitals. In vitro bactericidal activity was determined by a micromethod, using specific interfering substances over a range of dilutions, after 1, 3 and 5 min exposure times. A 5 log10 reduction of the challenge inoculum was considered as the criterion of efficacy. Any resistant strains were tested with the French Standard (T72300). When the micromethod was carried out at 20 degrees C, 10.7% (54/504) of the strains were resistant to the PVP-I skin disinfectant (dilution 1:10) and 1.6% (8/504) were resistant to the handwashing formulations (dilution 1:3) after 1 min exposure. By increasing the temperature to 32 degrees C, the resistance rate to the skin disinfectant fell to 1.9% (10/504). All of the 18 strains found resistant with the micromethod were sensitive using the French standard.
对从法国12家医院的医院感染中分离出的504株细菌菌株，测试了两种聚维酮碘（PVP - I）制剂，一种是抗菌洗手液，另一种是皮肤消毒剂。通过微量法测定体外杀菌活性，在一系列稀释度下，使用特定干扰物质，在暴露1、3和5分钟后进行测定。将挑战接种物减少5个对数10作为疗效标准。任何耐药菌株都按照法国标准（T72300）进行测试。当在20摄氏度下进行微量法时，暴露1分钟后，10.7%（54/504）的菌株对PVP - I皮肤消毒剂（稀释度1:10）耐药，1.6%（8/504）的菌株对洗手制剂（稀释度1:3）耐药。将温度提高到32摄氏度后，对皮肤消毒剂的耐药率降至1.9%（10/504）。通过微量法发现耐药的所有18株菌株，按照法国标准测试均敏感。
