Bussemas H H, Lippmann C, Schwedt G
J Clin Chem Clin Biochem. 1977 Jul;15(7):367-9.
The semiautomatic separation of the catecholamines, adrenaline and noradrenaline, is based on their adsorption onto aluminium oxide at pH 8.4, followed by batch elution with 0.05 mol/1 perchloric acid. The separate steps of the operation were investigated and optimised. Advantages of the method, compared with the manual column technique, are decreased time of operation, less involvement of personel, less danger of carry-over of interfering substances, and an exact standardisation of the processing time.
儿茶酚胺、肾上腺素和去甲肾上腺素的半自动分离是基于它们在pH 8.4时吸附到氧化铝上，然后用0.05 mol/1高氯酸进行分批洗脱。对该操作的各个步骤进行了研究和优化。与手动柱技术相比，该方法的优点是操作时间缩短、人员参与度降低、干扰物质残留的风险降低以及处理时间的精确标准化。
