• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

使用早熟凝集染色体的免疫金银染色法检测和评估高剂量γ射线诱导的染色体畸变

Detection and evaluation of chromosomal aberrations induced by high doses of gamma-irradiation using immunogold-silver painting of prematurely condensed chromosomes.

作者信息

Gotoh E, Asakawa Y

机构信息

Division of Genetic Resources, National Institute of Health Japan, Tokyo, Japan.

出版信息

Int J Radiat Biol. 1996 Nov;70(5):517-20. doi: 10.1080/095530096144716.

DOI:10.1080/095530096144716
PMID:8947532
Abstract

We developed a method for detecting chromosomal aberrations after irradiation with high doses of gamma-rays. At high dose levels, few of the irradiated cells are able to enter mitosis so that it is difficult to obtain enough mitotic chromosomes using a conventional colcemid block. Therefore, okadaic acid was used to condense prematurely the chromosomes of interphase cells. Even after irradiation with doses > 40 Gy, okadaic acid was able to force sufficient numbers of cells to condense their chromosomes. Segments of chromosomes were then detected by chromosome painting using a gold-conjugated antibody followed by silver enhancement. This simple method allows highly damaged chromosomes to be detected with great sensitivity.

摘要

我们开发了一种用于检测高剂量伽马射线照射后染色体畸变的方法。在高剂量水平下，很少有受照射细胞能够进入有丝分裂，因此使用传统的秋水仙酰胺阻滞法难以获得足够的有丝分裂染色体。因此，使用冈田酸使间期细胞的染色体过早浓缩。即使在照射剂量>40 Gy后，冈田酸仍能促使足够数量的细胞浓缩其染色体。然后使用金共轭抗体进行染色体描绘，接着进行银增强，以检测染色体片段。这种简单的方法能够高度灵敏地检测出严重受损的染色体。

相似文献

1
Detection and evaluation of chromosomal aberrations induced by high doses of gamma-irradiation using immunogold-silver painting of prematurely condensed chromosomes.使用早熟凝集染色体的免疫金银染色法检测和评估高剂量γ射线诱导的染色体畸变
Int J Radiat Biol. 1996 Nov;70(5):517-20. doi: 10.1080/095530096144716.
2
Simple biodosimetry method for use in cases of high-dose radiation exposure that scores the chromosome number of Giemsa-stained drug-induced prematurely condensed chromosomes (PCC).一种用于高剂量辐射暴露病例的简单生物剂量测定方法，该方法对吉姆萨染色的药物诱导早熟凝集染色体（PCC）的染色体数目进行计分。
Int J Radiat Biol. 2005 Jan;81(1):33-40. doi: 10.1080/09553000500092319.
3
Easy biodosimetry for high-dose radiation exposures using drug-induced, prematurely condensed chromosomes.利用药物诱导的早熟凝集染色体进行高剂量辐射暴露的简易生物剂量测定法。
Int J Radiat Biol. 1999 Apr;75(4):441-6. doi: 10.1080/095530099140366.
4
G2 Premature Chromosome Condensation/Chromosome Aberration Assay: Drug-Induced Premature Chromosome Condensation (PCC) Protocols and Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin for Radiation Biology.G2期染色体早熟凝集/染色体畸变分析：辐射生物学中用于有丝分裂染色体和间期染色质的药物诱导染色体早熟凝集（PCC）方案及细胞遗传学方法。
Methods Mol Biol. 2019;1984:47-60. doi: 10.1007/978-1-4939-9432-8_6.
5
Biodosimetry for high dose accidental exposures by drug induced premature chromosome condensation (PCC) assay.药物诱导的早熟染色体凝聚（PCC）试验在高剂量意外照射中的生物剂量测定。
Mutat Res. 2010 Jun 17;699(1-2):11-6. doi: 10.1016/j.mrgentox.2010.03.008. Epub 2010 Mar 23.
6
Suitability of scoring PCC rings and fragments for dose assessment after high-dose exposures to ionizing radiation.用于评估高剂量电离辐射暴露后剂量的 PCC 环和碎片的评分适用性。
Mutat Res. 2013 Sep 18;757(1):1-7. doi: 10.1016/j.mrgentox.2013.03.013. Epub 2013 Jul 19.
7
Biodosimetry of ionizing radiation by selective painting of prematurely condensed chromosomes in human lymphocytes.通过选择性标记人淋巴细胞中早凝染色体进行电离辐射的生物剂量测定。
Radiat Res. 1997 Nov;148(5 Suppl):S45-50.
8
Induction of premature chromosome condensation by a phosphatase inhibitor and a protein kinase in unstimulated human peripheral blood lymphocytes: a simple and rapid technique to study chromosome aberrations using specific whole-chromosome DNA hybridization probes for biological dosimetry.用磷酸酶抑制剂和蛋白激酶在未刺激的人外周血淋巴细胞中诱导早熟染色体凝集：一种使用特异性全染色体DNA杂交探针进行生物剂量测定来研究染色体畸变的简单快速技术。
Mutat Res. 2000 Mar 23;466(2):131-41. doi: 10.1016/s1383-5718(00)00011-5.
9
Detection of radiation-induced chromosome aberrations using fluorescence in situ hybridization in drug-induced premature chromosome condensations of tumour cell lines with different radiosensitivities.利用荧光原位杂交技术在具有不同放射敏感性的肿瘤细胞系药物诱导的早熟染色体凝缩中检测辐射诱导的染色体畸变。
Int J Radiat Biol. 1997 Mar;71(3):265-73. doi: 10.1080/095530097144148.
10
Chromosomal aberrations induced by low-dose gamma-irradiation. Study of R-banded chromosomes of human lymphocytes.低剂量γ射线照射诱导的染色体畸变。人类淋巴细胞R带染色体研究。
Mutat Res. 1989 Jun;212(2):167-72. doi: 10.1016/0027-5107(89)90068-7.

引用本文的文献

1
G-PCC-FISH derived multi-parametric biodosimetry methodology for accidental high dose and partial body exposures.基于 G-PCC-FISH 的多参数生物剂量测定方法，用于意外高剂量和部分身体照射。
Sci Rep. 2024 Jul 12;14(1):16103. doi: 10.1038/s41598-024-65330-8.
2
Visualizing Active Replication Regions in S-Phase Chromosomes.可视化 S 期染色体中的活跃复制区域。
Methods Mol Biol. 2023;2519:117-126. doi: 10.1007/978-1-0716-2433-3_14.
3
Chemical-Induced Premature Chromosome Condensation Protocol.化学诱导的染色体过早凝聚方案。
Methods Mol Biol. 2023;2519:41-51. doi: 10.1007/978-1-0716-2433-3_5.
4
A Basic and Simple Chromosome Preparation Protocol.一种基础且简易的染色体制备方案。
Methods Mol Biol. 2023;2519:1-7. doi: 10.1007/978-1-0716-2433-3_1.
5
Assessment of simulated high-dose partial-body irradiation by PCC-R assay.PCC-R 法评估模拟高剂量半身局部照射。
J Radiat Res. 2013 Sep;54(5):863-71. doi: 10.1093/jrr/rrt032. Epub 2013 Apr 17.
6
Visualizing the dynamics of chromosome structure formation coupled with DNA replication.可视化与DNA复制相关的染色体结构形成动态过程。
Chromosoma. 2007 Oct;116(5):453-62. doi: 10.1007/s00412-007-0109-5. Epub 2007 May 15.
7
Biodosimetry estimate for high-LET irradiation.高传能线密度辐射的生物剂量测定估计
Radiat Environ Biophys. 2007 Aug;46(3):229-35. doi: 10.1007/s00411-007-0110-7. Epub 2007 Apr 19.