Jackson M K, Ellis L C, Morrey J D, Li Z Z, Barnard D L
Department of Animal Sciences, Utah State University, Logan 84322, USA.
Am J Vet Res. 1996 Dec;57(12):1753-8.
To study temporal changes in amounts of viral DNA in blood leukocytes over long periods, and to determine whether severity of the disease is greater in experimentally induced, compared with natural, infection.
18 naturally and 6 experimentally infected black mink; 26 naturally infected brown mink.
Polymerase chain reaction amplification to detect viral DNA in blood and counter-immune electrophoresis to detect serum antibody were performed at regular intervals.
In naturally infected black mink, amounts of viral DNA were initially high, but after the appearance of antibody, viral DNA fluctuated and, in some instances, was undetectable. In other mink, small amounts of viral DNA were infrequently detected during the course of the infection. Amounts of viral DNA in leukocytes in late stages of the disease correlated with renal lesions in brown mink, but black mink had more severe lesions associated with smaller amounts of viral DNA. Severity of the disease was not enhanced in experimentally inoculated black mink.
After infection, leukocyte viral DNA is initially present in large amounts, but, in most mink, decreases markedly in association with the appearance of antibody. There is no difference in the progression and severity of the disease between black mink infected experimentally or naturally. Transmission of the disease may be enhanced by use of contaminated toenail clippers for blood collection.
长期研究血液白细胞中病毒DNA数量的时间变化，并确定与自然感染相比，实验性诱导感染时疾病的严重程度是否更高。
18只自然感染和6只实验感染的黑色水貂；26只自然感染的棕色水貂。
定期进行聚合酶链反应扩增以检测血液中的病毒DNA，并进行对流免疫电泳以检测血清抗体。
在自然感染的黑色水貂中，病毒DNA数量最初较高，但在抗体出现后，病毒DNA出现波动，在某些情况下无法检测到。在其他水貂中，在感染过程中偶尔检测到少量病毒DNA。疾病后期白细胞中的病毒DNA数量与棕色水貂的肾脏病变相关，但黑色水貂的病变更严重，而病毒DNA数量较少。实验接种的黑色水貂中疾病的严重程度并未增强。
感染后，白细胞病毒DNA最初大量存在，但在大多数水貂中，随着抗体的出现而显著减少。实验感染或自然感染的黑色水貂在疾病进展和严重程度上没有差异。使用受污染的趾甲剪进行采血可能会增加疾病传播。
