Mertens A H, Nagler J M, Galdermans D I, Slabbynck H R, Weise B S, Coolen D
Laboratory for Clinical Microbiology, Middelheim General Hospital, Antwerp, Belgium.
Eur J Clin Microbiol Infect Dis. 1996 Oct;15(10):807-10. doi: 10.1007/BF01701523.
The value of direct examination of Giemsa and Gram stains of cytospin preparations of protected specimen brush samples was compared to that of quantitative culture. Sixty-one samples from patients suspected to have nosocomial pneumonia were analysed. Twenty-five samples were positive by quantitative culture, 21 of which contained microorganisms seen by direct examination. The presence of leucocytes was not specific for a positive culture, but in their absence, a positive culture was unlikely. The presence of intracellular organisms always correlated with a positive culture, but was not very sensitive.
将保护样本刷检标本的细胞离心涂片吉姆萨染色和革兰氏染色直接检查的价值与定量培养的价值进行了比较。对61例疑似医院获得性肺炎患者的样本进行了分析。25份样本定量培养呈阳性，其中21份通过直接检查发现含有微生物。白细胞的存在对培养阳性并无特异性，但若无白细胞，则培养阳性的可能性不大。细胞内微生物的存在总是与培养阳性相关，但敏感性不高。
