Sekiya K, Danbara H, Yase K, Futaesaku Y
Department of Microbiology, School of Pharmaceutical Sciences, Kitasato University, Minato-ku, Tokyo, Japan.
J Bacteriol. 1996 Dec;178(23):6998-7002. doi: 10.1128/jb.178.23.6998-7002.1996.
The formation of pores by streptolysin O (SLO) was analyzed in erythrocyte membranes and liposomes by immunoelectron microscopy and electron spectroscopic imaging. The binding of SLO molecules to membranes was temperature independent, while the polymerization of SLO molecules was temperature dependent. Our results also suggest that proteins in erythrocyte membranes are not involved in the formation of SLO rings.
通过免疫电子显微镜和电子能谱成像技术,对链球菌溶血素O(SLO)在红细胞膜和脂质体中形成孔道的情况进行了分析。SLO分子与膜的结合不依赖于温度,而SLO分子的聚合则依赖于温度。我们的结果还表明,红细胞膜中的蛋白质不参与SLO环的形成。