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对链球菌溶血素O形成孔道两步理论的电子显微镜评估。

Electron microscopic evaluation of a two-step theory of pore formation by streptolysin O.

作者信息

Sekiya K, Danbara H, Yase K, Futaesaku Y

机构信息

Department of Microbiology, School of Pharmaceutical Sciences, Kitasato University, Minato-ku, Tokyo, Japan.

出版信息

J Bacteriol. 1996 Dec;178(23):6998-7002. doi: 10.1128/jb.178.23.6998-7002.1996.

DOI:10.1128/jb.178.23.6998-7002.1996
PMID:8955326
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC178605/
Abstract

The formation of pores by streptolysin O (SLO) was analyzed in erythrocyte membranes and liposomes by immunoelectron microscopy and electron spectroscopic imaging. The binding of SLO molecules to membranes was temperature independent, while the polymerization of SLO molecules was temperature dependent. Our results also suggest that proteins in erythrocyte membranes are not involved in the formation of SLO rings.

摘要

通过免疫电子显微镜和电子能谱成像技术,对链球菌溶血素O(SLO)在红细胞膜和脂质体中形成孔道的情况进行了分析。SLO分子与膜的结合不依赖于温度,而SLO分子的聚合则依赖于温度。我们的结果还表明,红细胞膜中的蛋白质不参与SLO环的形成。

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J Bacteriol. 1996 Dec;178(23):6998-7002. doi: 10.1128/jb.178.23.6998-7002.1996.
2
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本文引用的文献

1
The projection structure of perfringolysin O (Clostridium perfringens theta-toxin).产气荚膜梭菌θ毒素(产气荚膜梭菌溶素O)的投射结构。
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A ring-shaped structure with a crown formed by streptolysin O on the erythrocyte membrane.一种环形结构,在红细胞膜上有由链球菌溶血素O形成的冠。
J Bacteriol. 1993 Sep;175(18):5953-61. doi: 10.1128/jb.175.18.5953-5961.1993.
3
Different forms of streptolysin O produced by Streptococcus pyogenes and by Escherichia coli expressing recombinant toxin: cleavage by streptococcal cysteine protease.化脓性链球菌及表达重组毒素的大肠杆菌产生的不同形式的链球菌溶血素O:经链球菌半胱氨酸蛋白酶裂解
Infect Immun. 1995 Jul;63(7):2776-9. doi: 10.1128/iai.63.7.2776-2779.1995.
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Kinetics of streptolysin O self-assembly.链球菌溶血素O的自组装动力学
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Binding of cholesterol by sulfhydryl-activated cytolysins.巯基激活细胞溶素与胆固醇的结合
Infect Immun. 1980 Jan;27(1):97-101. doi: 10.1128/iai.27.1.97-101.1980.
6
Isolation and identification of two hemolytic forms of streptolysin-O.两种溶血形式的链球菌溶血素-O的分离与鉴定。
Infect Immun. 1984 Nov;46(2):394-400. doi: 10.1128/iai.46.2.394-400.1984.
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Streptococcal toxins.链球菌毒素
Rev Infect Dis. 1983 Sep-Oct;5 Suppl 4:S723-32. doi: 10.1093/clinids/5.supplement_4.s723.
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Interaction of streptolysin-O with biomembranes: kinetic and morphological studies on erythrocyte membranes.链球菌溶血素O与生物膜的相互作用:红细胞膜的动力学和形态学研究
Toxicon. 1985;23(3):425-39. doi: 10.1016/0041-0101(85)90026-1.
9
Mechanism of membrane damage by streptolysin-O.链球菌溶血素O造成膜损伤的机制。
Infect Immun. 1985 Jan;47(1):52-60. doi: 10.1128/iai.47.1.52-60.1985.
10
Use of a monoclonal antibody to determine the mode of transmembrane pore formation by streptolysin O.使用单克隆抗体确定链球菌溶血素O形成跨膜孔的方式。
Infect Immun. 1986 Dec;54(3):641-5. doi: 10.1128/iai.54.3.641-645.1986.