植物乳杆菌ldhL-ldhD双敲除菌株中葡萄糖和柠檬酸盐终产物的13C核磁共振分析
13C nuclear magnetic resonance analysis of glucose and citrate end products in an ldhL-ldhD double-knockout strain of Lactobacillus plantarum.
作者信息
Ferain T, Schanck A N, Delcour J
机构信息
Laboratoire de Génétique Moléculaire, Unité de Génétique, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.
出版信息
J Bacteriol. 1996 Dec;178(24):7311-5. doi: 10.1128/jb.178.24.7311-7315.1996.
Abstract
We have examined the metabolic consequences of knocking out the two ldh genes in Lactobacillus plantarum using 13C nuclear magnetic resonance. Unlike its wild-type isogenic progenitor, which produced lactate as the major metabolite under all conditions tested, ldh null strain TF103 mainly produced acetoin. A variety of secondary end products were also found, including organic acids (acetate, succinate, pyruvate, and lactate), ethanol, 2,3-butanediol, and mannitol.
摘要
我们利用13C核磁共振技术研究了植物乳杆菌中两个ldh基因敲除后的代谢后果。与其野生型同基因祖先不同，野生型在所有测试条件下都以乳酸作为主要代谢产物，而ldh基因缺失菌株TF103主要产生乙偶姻。还发现了多种次要终产物，包括有机酸（乙酸、琥珀酸、丙酮酸和乳酸）、乙醇）、乙醇、2,3-丁二醇和甘露醇。
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