用于检测曲霉半乳甘露聚糖的夹心酶联免疫吸附测定法的特异性
Specificity of a sandwich enzyme-linked immunosorbent assay for detecting Aspergillus galactomannan.
作者信息
Swanink C M, Meis J F, Rijs A J, Donnelly J P, Verweij P E
机构信息
Department of Medical Microbiology, University Hospital Nijmegen, The Netherlands.
出版信息
J Clin Microbiol. 1997 Jan;35(1):257-60. doi: 10.1128/jcm.35.1.257-260.1997.
Abstract
The specificity of a sandwich enzyme-linked immunosorbent assay (ELISA) for detecting Aspergillus galactomannan was tested with exoantigens of 29 fungi cultured from clinical specimens. Cross-reactivity was observed with Penicillium chrysogenum, Penicillium digitatum, and Paecilomyces variotii. Furthermore, 40 serum samples obtained from bacteremic patients with hematologic malignancies were retrospectively tested by sandwich ELISA. False-positive reactions with the serum were reproducible but did not correspond with the results of culture of specific microorganisms. Moreover, the microorganisms cultured from the blood showed no reactivity by the sandwich ELISA.
摘要
采用临床标本培养的29种真菌的外抗原，对检测曲霉半乳甘露聚糖的夹心酶联免疫吸附测定（ELISA）的特异性进行了测试。观察到与产黄青霉、指状青霉和拟青霉有交叉反应。此外，对40份血液系统恶性肿瘤菌血症患者的血清样本进行了夹心ELISA回顾性检测。血清的假阳性反应具有可重复性，但与特定微生物的培养结果不符。此外，从血液中培养出的微生物在夹心ELISA中无反应性。
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