用于诊断艾滋病相关中枢神经系统淋巴瘤和弓形虫病的多重聚合酶链反应
Multiplex PCR for diagnosis of AIDS-related central nervous system lymphoma and toxoplasmosis.
作者信息
Roberts T C, Storch G A
机构信息
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
出版信息
J Clin Microbiol. 1997 Jan;35(1):268-9. doi: 10.1128/jcm.35.1.268-269.1997.
Abstract
A nested multiplex PCR assay was designed for the simultaneous detection of Epstein-Barr virus and Toxoplasma gondii DNA from the cerebrospinal fluid of AIDS patients. T. gondii DNA was detected in 8 of 8 patients with Toxoplasma encephalitis and in 0 of 6 patients without toxoplasmosis, and Epstein-Barr virus DNA was found in 9 of 14 patients with central nervous system lymphoma and in 2 of 38 patients without disease.
摘要
设计了一种巢式多重聚合酶链反应检测方法，用于同时检测艾滋病患者脑脊液中的爱泼斯坦-巴尔病毒和弓形虫DNA。在8例弓形虫脑炎患者中，8例检测到弓形虫DNA，6例无弓形虫病的患者中，0例检测到该DNA；在14例中枢神经系统淋巴瘤患者中，9例检测到爱泼斯坦-巴尔病毒DNA，38例无该病的患者中，2例检测到该DNA。
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