Borriss R, Porwollik S, Schroeter R
Humboldt Universität Berlin, Germany.
Microbiology (Reading). 1996 Nov;142 ( Pt 11):3027-31. doi: 10.1099/13500872-142-11-3027.
Within the framework of the international project for sequencing the entire Bacillus subtilis genome, we have determined the complete sequence of the segment flanking the purE-D gene cluster (55 degrees) as far as cotA (52 degrees). This segment (34960 bp) contains, as well as 12 genes already identified as part of the pur operon, 17 putative ORFs and one partial one. Two of them (gabP and guaA) are known B. subtilis genes. The gene product of cotA (formerly pig) shows significant similarity to oxidoreductases (phenoxazine synthase and bilirubin oxidase). The putative products of ORFs yeaB (Czd protein), yeaC (MoxR), yebA (CNG-channel and cGMP-channel proteins from eukaryotes), yebB (hypothetical 32.9 kDa protein of Escherichia coli), yecA (amino acid permease) and yecB (adenine deaminase) were similar to proteins in data banks.
在对枯草芽孢杆菌全基因组进行测序的国际项目框架内，我们已确定了purE-D基因簇（55°）侧翼直至cotA（52°）的片段的完整序列。该片段（34960 bp）除了包含已被鉴定为嘌呤操纵子一部分的12个基因外，还包含17个推定的开放阅读框（ORF）和1个部分开放阅读框。其中两个（gabP和guaA）是已知的枯草芽孢杆菌基因。cotA（以前称为pig）的基因产物与氧化还原酶（吩恶嗪合酶和胆红素氧化酶）具有显著相似性。ORF yeaB（Czd蛋白）、yeaC（MoxR）、yebA（来自真核生物的CNG通道和cGMP通道蛋白）、yebB（大肠杆菌假定的32.9 kDa蛋白）、yecA（氨基酸通透酶）和yecB（腺嘌呤脱氨酶）的推定产物与数据库中的蛋白质相似。
