Rao S B, Sharma C P
Bio-Medical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojappura, India.
J Biomed Mater Res. 1997 Jan;34(1):21-8. doi: 10.1002/(sici)1097-4636(199701)34:1<21::aid-jbm4>3.0.co;2-p.
Chitosan, a mucopolysaccharide of marine origin, was studied for its safety and hemostatic potential. Its surface was treated with glutaraldehyde, carbodiimide, and plasma glow discharge to elicit effects of enzyme degradation. Of the seven enzymes used, leucine amino peptidase caused maximum degradation. Autoclaving appeared to be an ideal sterilizing method as it caused least decrease in tensile strength and effected a negligible rate of hemolysis. Sterilizing with glutaraldehyde with a physiologic pH retained the maximum tensile strength of chitosan. In vivo toxicity tests indicated that it is nontoxic, and the sterilized films were free of pyrogen. Coagulation and hemagglutination tests showed that the hemostatic mechanism of chitosan seems to be independent of the classical coagulation cascade and appears to be an interaction between the cell membrane of erythrocytes and chitosan.
壳聚糖是一种海洋来源的粘多糖，对其安全性和止血潜力进行了研究。用戊二醛、碳二亚胺和等离子体辉光放电处理其表面，以引发酶降解效应。在所使用的七种酶中，亮氨酸氨肽酶引起的降解最大。高压灭菌似乎是一种理想的灭菌方法，因为它导致的拉伸强度下降最小，溶血率可忽略不计。用生理pH值的戊二醛灭菌可保留壳聚糖的最大拉伸强度。体内毒性试验表明它无毒，且灭菌后的薄膜无热原。凝血和血细胞凝集试验表明，壳聚糖的止血机制似乎独立于经典的凝血级联反应，似乎是红细胞细胞膜与壳聚糖之间的相互作用。
