• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

白细胞介素1β对人正常及骨关节炎软骨中肌腱蛋白分布的影响：一项定量免疫组织化学研究。

Influence of interleukin 1 beta on tenascin distribution in human normal and osteoarthritic cartilage: a quantitative immunohistochemical study.

作者信息

Chevalier X, Claudepierre P, Groult N, Godeau G J

机构信息

Department of Rheumatology, Henri-Mondor hospital, Creteil, France.

出版信息

Ann Rheum Dis. 1996 Oct;55(10):772-5. doi: 10.1136/ard.55.10.772.

DOI:10.1136/ard.55.10.772
PMID:8984945
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1010298/
Abstract

OBJECTIVE

To determine the influence of IL-1 beta on the presence and the distribution of tenascin in matrix of human normal and osteoarthritic cartilage explants.

METHODS

Cartilage was grown in organotypic culture with or without IL-1 beta (10 ng ml1). Tenascin antigen was detected on cryopreserved cartilage sections by immunohistochemical techniques with a monoclonal antibody directed against all tenascin isoforms (BC-4), and then quantified by video imaging densitometry.

RESULTS

Tenascin was present in normal cartilage explants and increased in osteoarthritic cartilage explants. Treatment of normal and osteoarthritic cartilage explants with IL-1 beta (10 ng ml-1) induced an increase in tenascin content, which was particularly high in normal cartilage and predominated in the superficial layers of damaged cartilage. There was no obvious correlation between proteoglycan loss and presence of tenascin.

CONCLUSIONS

In human normal and osteoarthritic cartilage explants, the presence and the distribution of tenascin are influenced by IL-1 beta.

摘要

目的

确定白细胞介素-1β（IL-1β）对人正常及骨关节炎软骨外植体基质中肌腱蛋白的存在及分布的影响。

方法

将软骨在有或无IL-1β（10纳克/毫升）的条件下进行器官型培养。通过免疫组织化学技术，使用针对所有肌腱蛋白异构体的单克隆抗体（BC-4），在冷冻保存的软骨切片上检测肌腱蛋白抗原，然后通过视频成像密度测定法进行定量。

结果

肌腱蛋白存在于正常软骨外植体中，在骨关节炎软骨外植体中增加。用IL-1β（10纳克/毫升）处理正常和骨关节炎软骨外植体可导致肌腱蛋白含量增加，在正常软骨中尤其高，且在受损软骨的表层占主导。蛋白聚糖损失与肌腱蛋白的存在之间没有明显相关性。

结论

在人正常及骨关节炎软骨外植体中，肌腱蛋白的存在及分布受IL-1β影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6a9/1010298/dc6ffb462900/annrheumd00355-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6a9/1010298/dc6ffb462900/annrheumd00355-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6a9/1010298/dc6ffb462900/annrheumd00355-0081-a.jpg

相似文献

1
Influence of interleukin 1 beta on tenascin distribution in human normal and osteoarthritic cartilage: a quantitative immunohistochemical study.白细胞介素1β对人正常及骨关节炎软骨中肌腱蛋白分布的影响：一项定量免疫组织化学研究。
Ann Rheum Dis. 1996 Oct;55(10):772-5. doi: 10.1136/ard.55.10.772.
2
Tenascin distribution in articular cartilage from normal subjects and from patients with osteoarthritis and rheumatoid arthritis.
Arthritis Rheum. 1994 Jul;37(7):1013-22. doi: 10.1002/art.1780370706.
3
Tenascin and aggrecan expression by articular chondrocytes is influenced by interleukin 1beta: a possible explanation for the changes in matrix synthesis during osteoarthritis.关节软骨细胞的腱生蛋白和聚集蛋白聚糖表达受白细胞介素1β影响：骨关节炎期间基质合成变化的一种可能解释。
Ann Rheum Dis. 2004 Mar;63(3):240-4. doi: 10.1136/ard.2002.003749.
4
Presence and distribution of collagen II, collagen I, fibronectin, and tenascin in rabbit normal and osteoarthritic cartilage.兔正常及骨关节炎软骨中Ⅱ型胶原、Ⅰ型胶原、纤连蛋白和腱生蛋白的存在及分布
J Rheumatol. 1999 Feb;26(2):386-94.
5
Immunohistochemical detection and immunochemical analysis of type II collagen degradation in human normal, rheumatoid, and osteoarthritic articular cartilages and in explants of bovine articular cartilage cultured with interleukin 1.人正常、类风湿性和骨关节炎关节软骨以及用白细胞介素1培养的牛关节软骨外植体中II型胶原降解的免疫组织化学检测和免疫化学分析。
J Clin Invest. 1989 Feb;83(2):647-61. doi: 10.1172/JCI113929.
6
Distribution and role of tenascin-C in human osteoarthritic cartilage.肌腱蛋白-C在人骨关节炎软骨中的分布及作用
J Orthop Sci. 2010 Sep;15(5):666-73. doi: 10.1007/s00776-010-1513-x. Epub 2010 Oct 16.
7
Articular cartilage explant culture; an appropriate in vitro system to compare osteoarthritic and normal human cartilage.
Connect Tissue Res. 1993;29(4):287-99. doi: 10.3109/03008209309016834.
8
Tenascin-C induces inflammatory mediators and matrix degradation in osteoarthritic cartilage.Tenascin-C 诱导骨关节炎软骨中的炎症介质和基质降解。
BMC Musculoskelet Disord. 2011 Jul 15;12:164. doi: 10.1186/1471-2474-12-164.
9
The increased synthesis of inducible nitric oxide inhibits IL-1ra synthesis by human articular chondrocytes: possible role in osteoarthritic cartilage degradation.诱导型一氧化氮合成增加抑制人关节软骨细胞白细胞介素-1受体拮抗剂的合成：在骨关节炎软骨降解中的可能作用。
Osteoarthritis Cartilage. 1996 Mar;4(1):77-84. doi: 10.1016/s1063-4584(96)80009-4.
10
Local changes in proteoglycan synthesis during culture are different for normal and osteoarthritic cartilage.培养过程中蛋白聚糖合成的局部变化在正常软骨和骨关节炎软骨中有所不同。
Am J Pathol. 1992 Jun;140(6):1421-9.

引用本文的文献

1
The Role of Tenascin-C in the Physiopathology of Familial Mediterranean Fever.肌腱蛋白-C在家族性地中海热病理生理学中的作用
Cureus. 2024 Jul 8;16(7):e64067. doi: 10.7759/cureus.64067. eCollection 2024 Jul.
2
An investigation of the relationship between Behçet's disease and tenascin-C.白塞病与肌腱蛋白-C之间关系的研究。
Arch Rheumatol. 2024 Jan 15;39(1):107-114. doi: 10.46497/ArchRheumatol.2024.10163. eCollection 2024 Mar.
3
Tenascin-C induces inflammatory mediators and matrix degradation in osteoarthritic cartilage.

本文引用的文献

1
Tenascin distribution in articular cartilage from normal subjects and from patients with osteoarthritis and rheumatoid arthritis.
Arthritis Rheum. 1994 Jul;37(7):1013-22. doi: 10.1002/art.1780370706.
2
Stimulation by human interleukin 1 of cartilage breakdown and production of collagenase and proteoglycanase by human chondrocytes but not by human osteoblasts in vitro.在体外，人白细胞介素1刺激人软骨细胞导致软骨分解并产生胶原酶和蛋白聚糖酶，但不刺激人成骨细胞。
Biochim Biophys Acta. 1984 Feb 14;797(2):186-93. doi: 10.1016/0304-4165(84)90121-1.
3
Tenascin: an extracellular matrix protein prominent in specialized embryonic tissues and tumors.腱生蛋白：一种在特殊胚胎组织和肿瘤中显著存在的细胞外基质蛋白。
Tenascin-C 诱导骨关节炎软骨中的炎症介质和基质降解。
BMC Musculoskelet Disord. 2011 Jul 15;12:164. doi: 10.1186/1471-2474-12-164.
4
Tenascin and aggrecan expression by articular chondrocytes is influenced by interleukin 1beta: a possible explanation for the changes in matrix synthesis during osteoarthritis.关节软骨细胞的腱生蛋白和聚集蛋白聚糖表达受白细胞介素1β影响：骨关节炎期间基质合成变化的一种可能解释。
Ann Rheum Dis. 2004 Mar;63(3):240-4. doi: 10.1136/ard.2002.003749.
5
Early response genes induced in chondrocytes stimulated with the inflammatory cytokine interleukin-1beta.在受到炎性细胞因子白细胞介素-1β刺激的软骨细胞中诱导产生的早期反应基因。
Arthritis Res. 2001;3(6):381-8. doi: 10.1186/ar331. Epub 2001 Sep 18.
Annu Rev Cell Biol. 1989;5:71-92. doi: 10.1146/annurev.cb.05.110189.000443.
4
Cell adhesion to fibronectin and tenascin: quantitative measurements of initial binding and subsequent strengthening response.细胞与纤连蛋白和腱生蛋白的黏附：初始结合及后续强化反应的定量测定
J Cell Biol. 1989 Oct;109(4 Pt 1):1795-805. doi: 10.1083/jcb.109.4.1795.
5
Induction of tenascin in healing wounds.愈合伤口中肌腱蛋白的诱导生成。
J Cell Biol. 1988 Dec;107(6 Pt 2):2757-67. doi: 10.1083/jcb.107.6.2757.
6
The distribution of tenascin coincides with pathways of neural crest cell migration.腱生蛋白的分布与神经嵴细胞迁移途径一致。
Development. 1988 Jan;102(1):237-50. doi: 10.1242/dev.102.1.237.
7
The synthesis and accumulation of fibronectin by human articular cartilage.人关节软骨中纤连蛋白的合成与积累。
J Rheumatol. 1990 Jan;17(1):65-72.
8
The effects of interleukin-1 on the expression of thrombospondin and fibronectin by rabbit articular chondrocytes.白细胞介素-1对兔关节软骨细胞血小板反应蛋白和纤连蛋白表达的影响。
Exp Cell Res. 1991 Aug;195(2):462-7. doi: 10.1016/0014-4827(91)90397-d.
9
Focal adhesion integrity is downregulated by the alternatively spliced domain of human tenascin.纤连蛋白的可变剪接结构域可下调粘着斑的完整性。
J Cell Biol. 1991 Nov;115(4):1127-36. doi: 10.1083/jcb.115.4.1127.
10
Tenascin in normal, reactive, hyperplastic, and neoplastic tissues: biologic and pathologic implications.
Hum Pathol. 1991 Jul;22(7):636-43. doi: 10.1016/0046-8177(91)90285-w.