Tenreiro S, Nobre M F, Rainey F A, Miguel C, da Costa M S
Departamento de Bioquímica, Universidade de Coimbra, Portugal.
Int J Syst Bacteriol. 1997 Jan;47(1):122-6. doi: 10.1099/00207713-47-1-122.
Six slightly halophilic, thermophilic bacterial strains were isolated from saline hot springs along the Bay of Naples, Italy. These strains produce bright yellow colonies and have a filamentous morphology and an optimum growth temperature of about 60 degrees C. Lipid composition and 16S ribosomal DNA sequence analyses showed that these strains belong to the genus Thermonema, a member of the cytophaga-flavobacter-bacteroides phylum. Growth was observed in medium containing 1 to 3% NaCl. The DNA G + C content was 50.9 mol%. DNA-DNA hybridization studies showed that these strains represent a new species of the genus Thermonema. We propose that strain NR-27T (T = type strain) and the other strains represent a new species, Thermonema rossianum. Strain NR-27 (= DSM 10300) is the type strain of this species.
从意大利那不勒斯湾沿岸的盐温泉中分离出了6株轻度嗜盐嗜热细菌菌株。这些菌株形成亮黄色菌落，具有丝状形态，最适生长温度约为60摄氏度。脂质组成和16S核糖体DNA序列分析表明，这些菌株属于嗜热放线菌属，是噬细胞-黄杆菌-拟杆菌门的成员。在含有1%至3%氯化钠的培养基中观察到生长。DNA的G + C含量为50.9摩尔%。DNA-DNA杂交研究表明，这些菌株代表嗜热放线菌属的一个新物种。我们提议将菌株NR-27T（T = 模式菌株）及其他菌株代表一个新物种，即罗氏嗜热放线菌。菌株NR-27（= DSM 10300）是该物种的模式菌株。
