Drancourt M, Bollet C, Raoult D
Unité des Rickettsies CNRS EP J0054, Faculté de Médicine, Marseille, France.
Int J Syst Bacteriol. 1997 Jan;47(1):160-3. doi: 10.1099/00207713-47-1-160.
A gram-negative bacterium was isolated from a cerebrospinal fluid sample from an HIV-seropositive Rwandan refugee with primary meningoencephalitis. This Marseille-Goma sample B isolate, strain MGBT (T = type strain), was found to exhibit evolutionary homology with Stenotrophomonas maltophilia, as determined by a 16S rRNA gene sequence analysis, and this finding was reflected by similar phenotypic traits. MGBT could, however, be distinguished from the S. maltophilia type strain by using a number of biochemical and physiological tests, and a genotypic analysis of the two strains in which DNA homology was used revealed only 35% homology between them. Furthermore, the antibiotic susceptibility of MGBT was restricted to netilmicin, ciprofloxacin, trimethoprim-sulfamethoxazole, and colimycin. On the basis of these results we propose that MGBT is a representative of a new species in the genus Stenotrophomonas, Stenotrophomonas africana.
从一名患有原发性脑膜脑炎的艾滋病毒血清阳性卢旺达难民的脑脊液样本中分离出一种革兰氏阴性细菌。通过16S rRNA基因序列分析确定，这种马赛-戈马样本B分离株，即菌株MGBT（T = 模式菌株），与嗜麦芽窄食单胞菌具有进化同源性，这一发现也体现在相似的表型特征上。然而，通过一些生化和生理测试，MGBT可与嗜麦芽窄食单胞菌模式菌株区分开来，并且使用DNA同源性对这两种菌株进行的基因型分析显示它们之间只有35%的同源性。此外，MGBT的抗生素敏感性仅限于奈替米星、环丙沙星、甲氧苄啶-磺胺甲恶唑和黏菌素。基于这些结果，我们提出MGBT是嗜麦芽窄食单胞菌属中的一个新物种——非洲嗜麦芽窄食单胞菌的代表。
