Xiong Q, Kadota S, Tani T, Namba T
Research Institute for Wakan-Yaku (Traditional Sino-Japanese Medicines), Toyama Medical and Pharmaceutical University, Japan.
Biol Pharm Bull. 1996 Dec;19(12):1580-5. doi: 10.1248/bpb.19.1580.
The acetone-H2O (9:1) extract from the stem of Cistanche deserticola showed a strong free radical scavenging activity. Nine major phenylethanoid compounds were isolated from this extract. They were identified by NMR as acteoside, isoacteoside, 2'-acetylacteoside, tubuloside B, echinacoside, tubuloside A, syringalide A 3'-alpha-rhamnopyranoside, cistanoside A and cistanoside F. All of these compounds showed stronger free radical scavenging activities than alpha-tocopherol on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and xanthine/xanthine oxidase (XOD) generated superoxide anion radical (O2-.). Among the nine compounds, isoacteoside and tubuloside B, whose caffeoyl moiety is at 6'-position of the glucose, showed an inhibitory effect on XOD. We further studied the effects of these phenylethanoids on the lipid peroxidation in rat liver microsomes induced by enzymatic and non-enzymatic methods. As expected, each of them exhibited significant inhibition on both ascorbic acid/Fe2+ and ADP/NADPH/Fe3+ induced lipid peroxidation in rat liver microsomes, which were more potent than alpha-tocopherol of caffeic acid. The antioxidative effect was found to be potentiated by an increase in the number of phenolic hydroxyl groups in the molecule.
肉苁蓉茎的丙酮 - 水（9:1）提取物表现出较强的自由基清除活性。从该提取物中分离出9种主要的苯乙醇类化合物。通过核磁共振鉴定它们为松果菊苷、异松果菊苷、2'-乙酰松果菊苷、管花苷B、紫锥菊苷、管花苷A、丁香脂素A 3'-α-鼠李糖苷、肉苁蓉苷A和肉苁蓉苷F。所有这些化合物在1,1-二苯基-2-苦基肼（DPPH）自由基和黄嘌呤/黄嘌呤氧化酶（XOD）产生的超氧阴离子自由基（O2-·）方面表现出比α-生育酚更强的自由基清除活性。在这9种化合物中，咖啡酰基位于葡萄糖6'-位的异松果菊苷和管花苷B对XOD有抑制作用。我们进一步研究了这些苯乙醇类化合物对酶法和非酶法诱导的大鼠肝微粒体脂质过氧化的影响。正如预期的那样，它们各自对大鼠肝微粒体中抗坏血酸/Fe2+和ADP/NADPH/Fe3+诱导的脂质过氧化均表现出显著抑制作用，且比咖啡酸的α-生育酚更有效。发现抗氧化作用会因分子中酚羟基数量的增加而增强。
