Rodríguez-García M I, Fernández J A, Rodríguez A, Fernández M P, Gutierrez C, Torre-Alonso J C
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Oviedo, Spain.
Ann Rheum Dis. 1996 Dec;55(12):895-900. doi: 10.1136/ard.55.12.895.
To investigate the occurrence of anti-annexin V autoantibodies in sera of patients with rheumatoid arthritis to assess involvement with the disease and any relation to glucocorticoid treatment.
Anti-annexin V antibodies were measured by an enzyme linked immunosorbent assay (ELISA) which used the purified human recombinant protein as antigen.
Concentrations of anti-annexin V autoantibodies, predominantly of the IgG class, were significantly raised in sera from patients with rheumatoid arthritis compared to normal controls. This was not correlated with other indices of disease activity such as erythrocyte sedimentation rate or C reactive protein and was unrelated to glucocorticoid treatment.
Extracellular annexin V provides an antigenic stimulus for autoantibody production and its in vivo expression is independent of glucocorticoid control. Such autoantibodies may have a detrimental role in the arthritic condition by interfering with putative functions of annexin V, including collagen type II binding, inhibition of phospholipase A2 activity, and Fc receptor activity.
研究类风湿关节炎患者血清中抗膜联蛋白V自身抗体的发生情况，以评估其与疾病的相关性以及与糖皮质激素治疗的任何关系。
采用以纯化的人重组蛋白为抗原的酶联免疫吸附测定（ELISA）法检测抗膜联蛋白V抗体。
与正常对照组相比，类风湿关节炎患者血清中抗膜联蛋白V自身抗体浓度显著升高，主要为IgG类。这与红细胞沉降率或C反应蛋白等其他疾病活动指标无关，且与糖皮质激素治疗无关。
细胞外膜联蛋白V为自身抗体产生提供抗原刺激，其在体内的表达独立于糖皮质激素控制。此类自身抗体可能通过干扰膜联蛋白V的假定功能（包括与II型胶原结合、抑制磷脂酶A2活性和Fc受体活性）在关节炎病情中发挥有害作用。
