Gorgels T G, van Beek L, van Norren D
F.C. Donders Institute of Ophthalmology, Utrecht Academic Hospital, The Netherlands.
Microsc Res Tech. 1997 Jan 15;36(2):89-95. doi: 10.1002/(SICI)1097-0029(19970115)36:2<89::AID-JEMT2>3.0.CO;2-Q.
Retinal light damage is influenced by the body temperature during exposure, but previous studies do not agree on the magnitude of the effect. A study in rats with broadband green light reported a much larger effect than a study with 380 nm light. The present study set out to clarify whether the spectral composition of the damaging light is responsible for this discrepancy by using 488 nm instead of 380 nm light. Wavelengths in the range of 470-550 nm are known to produce a different damage type than 380 nm. Small patches of retina of anesthetized rats were exposed to 488 nm radiation of an argon ion laser. Body temperature was varied between 30 and 40.5 degrees C. Three days after irradiation, the retina was inspected by funduscopy and prepared for light microscopy. The dose of radiation needed for a just visible change in fundo decreased by 6% per degree increase in body temperature. Damage was mainly found in the retinal pigment epithelium. Temperature had no effect on damage morphology. The temperature effect at 488 nm is much smaller than reported for broadband green light. We conclude that the spectral composition of the damaging light is not responsible for the discrepancy on the magnitude of the temperature effect. Other differences between the studies must be responsible, such as difference in retinal irradiance levels.
视网膜光损伤受暴露期间体温的影响，但先前的研究在该效应的程度上并未达成一致。一项针对大鼠的宽带绿光研究报告的效应比一项使用380纳米光的研究大得多。本研究旨在通过使用488纳米而非380纳米的光来阐明损伤光的光谱组成是否是造成这种差异的原因。已知470 - 550纳米范围内的波长会产生与380纳米不同类型的损伤。将麻醉大鼠的小块视网膜暴露于氩离子激光的488纳米辐射下。体温在30至40.5摄氏度之间变化。照射三天后，通过眼底镜检查视网膜并制备用于光学显微镜检查。眼底刚刚可见变化所需的辐射剂量随体温每升高一度降低6%。损伤主要发生在视网膜色素上皮。温度对损伤形态没有影响。488纳米处的温度效应远小于宽带绿光的报道。我们得出结论，损伤光的光谱组成不是造成温度效应程度差异的原因。研究之间的其他差异，如视网膜辐照度水平的差异，必定是造成这种差异的原因。
