Depuydt C E, Bosmans E, Zalata A, Schoonjans F, Comhaire F H
University Hospital Ghent, Department of Internal Medicine, Belgium.
J Androl. 1996 Nov-Dec;17(6):699-707.
The presence of various cytokines, namely hepatocyte growth factor (HGF), interleukin-1 receptor antagonist (IL-1 RA), and interleukins (IL-1 alpha, IL-6, and IL-8), as well as the production of reactive oxygen species (ROS) was investigated in seminal plasma of fertile and infertile patients in order to evaluate the possible value of measuring these substances for the diagnosis of male accessory gland infection, and to assess the possible relationship between oxidative stress and cytokines during leucocytospermia and male accessory gland infection (MAGI). Our findings indicate that all of the measured cytokines seem to be produced locally as well as by white blood cells (WBC) and that, due to the presence of higher numbers of WBC, accessory gland infection may exert a deleterious effect on sperm quality through the production of ROS and/or of particular cytokines such as IL-1 alpha, IL-1 RA, and IL-8. The most specific marker for a sensitivity of 95% in discriminating between cases with or without MAGI is the measurement of IL-6 in seminal plasma. In the absence of WBC several cytokines are constitutively produced and correlate with sperm concentration (HGF, IL-8), alpha-glucosidase (IL-6), and gamma-glutamyltransferase activity (HGF). The measurement of these cytokines in semen may provide clinically useful information for the diagnosis of male accessory gland infection, as well as in the absence of WBC where it can provide information about certain mechanisms of male reproductive function and dysfunction.
为了评估检测这些物质对诊断男性附属性腺感染的可能价值，并评估白细胞精子症和男性附属性腺感染（MAGI）期间氧化应激与细胞因子之间的可能关系，我们对有生育能力和无生育能力患者的精浆中各种细胞因子（即肝细胞生长因子（HGF）、白细胞介素-1受体拮抗剂（IL-1 RA）和白细胞介素（IL-1α、IL-6和IL-8））的存在情况以及活性氧（ROS）的产生进行了研究。我们的研究结果表明，所有检测的细胞因子似乎都是在局部以及由白细胞（WBC）产生的，并且由于白细胞数量较多，附属性腺感染可能通过产生ROS和/或特定细胞因子（如IL-1α、IL-1 RA和IL-8）对精子质量产生有害影响。在区分有无MAGI的病例时，精浆中IL-6的检测是敏感性为95%的最特异性标志物。在没有白细胞的情况下，几种细胞因子是组成性产生的，并且与精子浓度（HGF、IL-8）、α-葡萄糖苷酶（IL-6）和γ-谷氨酰转移酶活性（HGF）相关。精液中这些细胞因子的检测可能为男性附属性腺感染的诊断提供临床有用信息，以及在没有白细胞的情况下，它可以提供有关男性生殖功能和功能障碍某些机制的信息。
