大豆β-葡聚糖激发子结合蛋白的结构与功能
The structure and function of a soybean beta-glucan-elicitor-binding protein.
作者信息
Umemoto N, Kakitani M, Iwamatsu A, Yoshikawa M, Yamaoka N, Ishida I
机构信息
Central Laboratories for Key Technology, Kirin Brewery Co., Ltd., Fukuura, Kanazawa-ku, Yokohama, Japan.
出版信息
Proc Natl Acad Sci U S A. 1997 Feb 4;94(3):1029-34. doi: 10.1073/pnas.94.3.1029.
Abstract
beta-Glucan elicitor (GE), released from the cell wall of the phytopathogenic fungus Phytophthora megasperma by soybean glucanases, causes defense reactions in soybean. A GE-binding protein (GEBP) was purified from the membrane fraction of soybean root cells, and its cDNA was isolated. Expression of the cDNA clone in tobacco suspension cultured cells and in Escherichia coli conferred GE-binding activity to both. An antibody against the recombinant protein was found to inhibit the GE binding with the soybean cotyledon membrane fraction as well as the resulting accumulation of phytoalexin. Immunolocalization assays indicated that the GEBPs are located in the plasma membrane of root cells. These results suggest that the cDNA encodes a GE receptor and may mediate the signaling of the elicitor.
摘要
由大豆葡聚糖酶从致病疫霉细胞壁释放的β-葡聚糖激发子（GE）可引发大豆的防御反应。从大豆根细胞的膜组分中纯化出一种GE结合蛋白（GEBP），并分离出其cDNA。该cDNA克隆在烟草悬浮培养细胞和大肠杆菌中的表达均赋予了两者GE结合活性。发现针对重组蛋白的抗体可抑制GE与大豆子叶膜组分的结合以及由此导致的植保素积累。免疫定位分析表明，GEBPs位于根细胞的质膜中。这些结果表明，该cDNA编码一种GE受体，并可能介导激发子的信号传导。
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本文引用的文献
1
2
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Plant Physiol. 1990 Jun;93(2):673-82. doi: 10.1104/pp.93.2.673.
3
Phytoalexin Elicitor Activity of Carbohydrates from Phytophthora megasperma f.sp. glycinea and Other Sources.来自大豆疫霉大豆专化型及其他来源的碳水化合物的植保素诱导活性
Plant Physiol. 1983 Mar;71(3):466-71. doi: 10.1104/pp.71.3.466.
4
Release of a Soluble Phytoalexin Elicitor from Mycelial Walls of Phytophthora megasperma var. sojae by Soybean Tissues.大豆组织诱导大豆疫霉游动孢子囊壁释放可溶性激发子
Plant Physiol. 1981 May;67(5):1032-5. doi: 10.1104/pp.67.5.1032.
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Plant Physiol. 1978 Mar;61(3):314-7. doi: 10.1104/pp.61.3.314.
6
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Plant Physiol. 1976 May;57(5):751-9. doi: 10.1104/pp.57.5.751.
7
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8
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Annu Rev Plant Physiol Plant Mol Biol. 1997 Jun;48:251-275. doi: 10.1146/annurev.arplant.48.1.251.
9
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Plant Physiol. 1994 Feb;104(2):683-689. doi: 10.1104/pp.104.2.683.
10
Systematic peptide fragmentation of polyvinylidene difluoride(PVDF)-immobilized proteins prior to microsequencing.在进行微量测序之前，对固定在聚偏二氟乙烯（PVDF）上的蛋白质进行系统性肽段裂解。
J Biochem. 1996 Jul;120(1):29-34. doi: 10.1093/oxfordjournals.jbchem.a021389.
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