Parra B, Slots J
Department of Periodontology, School of Dentistry, University of Southern California, Los Angeles 90089-0641, USA.
Oral Microbiol Immunol. 1996 Oct;11(5):289-93. doi: 10.1111/j.1399-302x.1996.tb00183.x.
Even though viruses have been implicated in the etiology of several medical and dental disorders, little or no data are available on the possible involvement of human viruses in the pathogenesis of human periodontal disease. This study investigated the presence of human cytomegalovirus, Epstein-Barr virus, herpes simplex virus, human papillomavirus and human immunodeficiency virus (HIV) in crevicular fluid samples from 30 patients with advanced periodontitis and 26 subjects with gingivitis. Viral identification was performed on direct subgingival samples from 3 diseased sites in each patient using the polymerase chain reaction technique. Seventy-eight percent of advanced periodontitis patients were positive for at least one of the five test viruses. Cytomegalovirus was detected in 60% of the periodontitis patients, Epstein-Barr virus in 30%, herpes simplex virus in 20%, human papillomavirus in 17% and HIV in 7%. Forty percent of the periodontitis patients revealed coinfection by 2 to 5 viruses. Only 31% of the gingivitis subjects showed a positive viral identification in crevicular fluid, and infected individuals only revealed human cytomegalovirus. This study demonstrated that human viruses may occur in periodontitis lesions with relatively high prevalence. The pathogenetic significance of human viruses in destructive periodontal disease needs to be determined.
尽管病毒已被认为与多种医学和牙科疾病的病因有关，但关于人类病毒可能参与人类牙周病发病机制的数据却很少或几乎没有。本研究调查了30例晚期牙周炎患者和26例牙龈炎患者龈沟液样本中人类巨细胞病毒、爱泼斯坦-巴尔病毒、单纯疱疹病毒、人乳头瘤病毒和人类免疫缺陷病毒（HIV）的存在情况。使用聚合酶链反应技术对每位患者3个患病部位的直接龈下样本进行病毒鉴定。78%的晚期牙周炎患者至少对五种检测病毒中的一种呈阳性。在60%的牙周炎患者中检测到巨细胞病毒，30%检测到爱泼斯坦-巴尔病毒，20%检测到单纯疱疹病毒，17%检测到人乳头瘤病毒，7%检测到HIV。40%的牙周炎患者显示同时感染2至5种病毒。只有31%的牙龈炎受试者龈沟液病毒鉴定呈阳性，且感染个体仅检测到人类巨细胞病毒。本研究表明，人类病毒可能以相对较高的患病率出现在牙周炎病变中。人类病毒在破坏性牙周病中的致病意义有待确定。
