Rakotoarison D A, Gressier B, Trotin F, Brunet C, Dine T, Luyckx M, Vasseur J, Cazin M, Cazin J C, Pinkas M
Laboratoire de Pharmacologie, Pharmacocinétique et Pharmacie Clinique, Université des Sciences et Technologies de Lille, Villeneuve d'Ascq, France.
Pharmazie. 1997 Jan;52(1):60-4.
Numerous plants synthesize among their secondary metabolites phenolic compounds which possess antioxidant effects. The aim of the present work was to assay the antioxidant activities of phenolics from Crataegus monogyna Jacq. flowers and in vitro tissue culture (calli and cell suspensions) extracts. In the case of tissue culture extracts, the phenolic production is studied at three different stages of one subculture period (initial growth period, increasing and maximal phenolic synthesis phases). Attention was paid to the main categories: flavonoids and proanthocyanidins, and to the principal individual components. Total phenolic amounts decrease in the order: fresh flowers > cell suspension cultures > callus cultures. The antioxidant activities of these different extracts against H2O2 and HOCl, have been determined in vitro. All the extracts are efficient and the scavenging capacity is clearly related to the total phenol content. The scavenging effects of the cell suspension extracts are similar to those of the flowers. Among individual compounds, the flavanol-type derivatives, specially the proanthocyanidin B2, are more efficient. Thus, in vitro plant tissues could be an interesting source of bioactive molecules.
许多植物在其次级代谢产物中合成具有抗氧化作用的酚类化合物。本研究的目的是测定山楂花以及体外组织培养（愈伤组织和细胞悬浮液）提取物中酚类物质的抗氧化活性。对于组织培养提取物，在一个继代培养周期的三个不同阶段（初始生长期、酚类合成增加期和最大合成期）研究酚类物质的产生情况。研究重点关注主要类别：黄酮类化合物和原花青素，以及主要的单一成分。总酚含量按以下顺序降低：鲜花＞细胞悬浮培养物＞愈伤组织培养物。已在体外测定了这些不同提取物对过氧化氢和次氯酸的抗氧化活性。所有提取物都具有高效性，清除能力与总酚含量明显相关。细胞悬浮液提取物的清除效果与鲜花的类似。在单一化合物中，黄烷醇型衍生物，特别是原花青素B2，更具高效性。因此，体外植物组织可能是生物活性分子的一个有趣来源。
