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紫外线探测器孢子膜的特性与应用:一种新型探测器系统的灵敏度曲线与紫外线诱发人体皮肤红斑的作用光谱具有良好的相似性。

Characterization and application of UV detector spore films: the sensitivity curve of a new detector system provides good similarity to the action spectrum for UV-induced erythema in human skin.

作者信息

Quintern L E, Furusawa Y, Fukutsu K, Holtschmidt H

机构信息

BioSense, Biosensory Systems, Bornheim, Germany.

出版信息

J Photochem Photobiol B. 1997 Jan;37(1-2):158-66. doi: 10.1016/s1011-1344(96)04414-4.

DOI:10.1016/s1011-1344(96)04414-4
PMID:9043103
Abstract

The UV action spectra of two different biologically weighting UV photofilms (spore films), produced with Bacillus subtilis spores (wild-type and DNA repair-deficient strains), were determined at the Okasaki large spectrograph (OLS) within the level of wavelength range 254-400 nm. The action spectrum of the mutant strain film was modified with a cut-off filter, yielding a sensitivity curve similar to the action spectrum for erythemal induction in human skin. The detector system was tested in a field study and in a study using lamps with different UV spectral compositions. The system demonstrated its applicability over the spectral region lambda = 290 nm to the visible light. The system could be calibrated to give the minimal erythemal dose.

摘要

利用枯草芽孢杆菌孢子(野生型和DNA修复缺陷型菌株)制备了两种不同的生物加权紫外光胶片(孢子胶片),在冈崎大型光谱仪(OLS)上测定了波长范围为254 - 400 nm的紫外作用光谱。用截止滤光片对突变菌株胶片的作用光谱进行了修正,得到了一条与人体皮肤红斑诱导作用光谱相似的灵敏度曲线。该检测系统在一项现场研究和一项使用不同紫外光谱组成灯具的研究中进行了测试。该系统在波长λ = 290 nm至可见光的光谱区域展示了其适用性。该系统可以校准以给出最小红斑剂量。

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