Korth K L, Stermer B A, Bhattacharyya M K, Dixon R A
The Samuel Roberts Noble Foundation, Plant Biology Division, Ardmore, OK 73402, USA.
Plant Mol Biol. 1997 Feb;33(3):545-51. doi: 10.1023/a:1005743011651.
We isolated two full-length cDNA clones encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) from potato (Solanum tuberosum) L. tubers. The clones, designated hmg2.2 and hmg3.3, are members of previously described gene subfamilies. In addition to being induced by arachidonic acid in tubers, hmg2.2 transcript accumulates developmentally in young flowers, and in mature sepals and ovaries, whereas transcript for hmg3.3 accumulates in mature petals and anthers. Our data suggest that members of specific HMGR-encoding gene subfamilies might be involved in both defense responses and flower development. Accumulation of different HMGR transcripts could provide some control of isoprenoid biosynthesis by producing isoforms specific for classes of end-products produced in particular tissues.
我们从马铃薯（Solanum tuberosum）块茎中分离出两个编码3-羟基-3-甲基戊二酰辅酶A还原酶（HMGR）的全长cDNA克隆。这两个克隆分别命名为hmg2.2和hmg3.3，它们属于先前描述的基因亚家族成员。除了在块茎中受花生四烯酸诱导外，hmg2.2转录本在幼花、成熟萼片和子房的发育过程中积累，而hmg3.3的转录本则在成熟花瓣和花药中积累。我们的数据表明，特定的编码HMGR基因亚家族成员可能参与防御反应和花的发育。不同HMGR转录本的积累可能通过产生特定组织中特定终产物类别的同工型，对类异戊二烯生物合成进行某种控制。
