Freeman R, Sisson P R, Barer M R, Ward A C, Lightfoot N F
Public Health Laboratory, Newcastle General Hospital, Newcastle-upon-Tyne, UK.
Zentralbl Bakteriol. 1997 Jan;285(2):285-90. doi: 10.1016/s0934-8840(97)80036-9.
The ability of pyrolysis mass spectrometry (Py-MS) to discriminate between paired groups of closely related bacteria has been examined. The technique was challenged with increasing levels of biological difference; from that between different subcultures of the same isolate of Staphylococcus aureus, through that between separate isolates of the same strain and eventually to that between different staphylococcal species and Streptococcus pyogenes. By using an analytical method in which the spectral data from any two groups can be directly compared at a time, it was shown that Py-MS was capable of measuring statistically significant differences between staphylococci and Streptococcus pyogenes, between different Staphylococcus species and between two isolates of the same strain of S. aureus from different sources. Two subcultures of the same isolate were found to be indistinguishable. The differing magnitude of the Py-MS-derived differences corresponded to the "biological differences", being much larger between staphylococci and streptococci than between staphylococcal species and only just statistically significant between isolates of the same strain from different sources. The technique described allows the assessment of the magnitude and significance of Py-MS-derived differences between any two bacterial populations.
对热解质谱法（Py-MS）区分密切相关细菌配对组的能力进行了研究。该技术面临着日益增加的生物学差异挑战；从同一株金黄色葡萄球菌不同传代培养物之间的差异，到同一菌株不同分离株之间的差异，最终到不同葡萄球菌属物种与化脓性链球菌之间的差异。通过使用一种可以一次直接比较任意两组光谱数据的分析方法，结果表明Py-MS能够测量葡萄球菌与化脓性链球菌之间、不同葡萄球菌属物种之间以及来自不同来源的同一株金黄色葡萄球菌的两个分离株之间具有统计学意义的差异。发现同一分离株的两种传代培养物无法区分。Py-MS衍生差异的不同幅度与“生物学差异”相对应，葡萄球菌与链球菌之间的差异远大于葡萄球菌属物种之间的差异，且仅在来自不同来源的同一菌株分离株之间具有统计学意义。所描述的技术能够评估Py-MS衍生的任意两个细菌群体之间差异的大小和显著性。
