Mathers K, Freeman R, Sisson P R, Lightfoot N F
Department of Microbiology, Medical School, Newcastle-upon-Tyne, UK.
Zentralbl Bakteriol. 1997 Jan;285(2):311-8. doi: 10.1016/s0934-8840(97)80040-0.
Whole cell samples from cultures of Staphylococcus aureus, S. hominis, S. epidermidis and Streptococcus pyogenes were analysed by pyrolysis mass spectrometry and the results were compared with Py-MS-derived analyses of samples of DNA extracted from the same organisms. Py-MS analysis differentiated the four organisms in both circumstances. These results challenge previous assumptions that Py-MS is restricted to detecting phenotypic differences, although the basis for the differentiation of the DNA extracts has yet to be determined.
对金黄色葡萄球菌、人葡萄球菌、表皮葡萄球菌和化脓性链球菌培养物的全细胞样品进行了热解质谱分析，并将结果与从相同生物体中提取的DNA样品的热解质谱分析结果进行了比较。在这两种情况下，热解质谱分析都能区分这四种生物体。这些结果挑战了先前认为热解质谱仅限于检测表型差异的假设，尽管DNA提取物的区分基础尚未确定。
