Kirstein F, Rijpkema S, Molkenboer M, Gray J S
University College Dublin, Belfield, Ireland.
Eur J Epidemiol. 1997 Jan;13(1):67-72. doi: 10.1023/a:1007360422975.
Questing Ixodes ricinus ticks were collected from six locations throughout Ireland and 638 nymphs, 111 females and 118 males were investigated for infection with Borrelia burgdorferi sensulato (s.l.). The total prevalence of B. burgdorferi s.l. in the ticks was determined as 14.9% by polymerase chain reaction (PCR) amplification of the spacer region of 5S-23S rRNA genes. Infection prevalence was significantly higher in adult (20.1%) Ixodes ricinus compared to nymphs (13.1%). The prevalence of infection in adult male and female ticks was similar (19.5% and 20.7% respectively). The genomospecies B. burgdorferi sensu stricto, B. afzelii, B. garinii and group VS116 were identified by reverse line blot (RLB) using genomospecies specific oligonucleotide probes. The most prevalent B. burgdorferi genomospecies identified were VS116 (34.6%), B. garinii (24.3%) and B. burgdorferi sensu stricto (18.4%). B. afzelii was uncommon (6.6%). Multiple infections were observed in 13.2% of the infected ticks. The distribution of the genomospecies showed geographical variation and also seemed to be influenced by the nature of the habitat. A broad range of genomospecies seemed to be associated with the presence of a wide spectrum of potential reservoir hosts in the habitat and also with a high overall prevalence of B. burgdorferi s.l.
在爱尔兰各地的六个地点采集了饥饿的蓖麻硬蜱，并对638只若虫、111只雌蜱和118只雄蜱进行了伯氏疏螺旋体狭义种（Borrelia burgdorferi sensulato，s.l.）感染情况调查。通过对5S - 23S rRNA基因间隔区进行聚合酶链反应（PCR）扩增，确定蜱中伯氏疏螺旋体狭义种的总感染率为14.9%。蓖麻硬蜱成虫（20.1%）的感染率显著高于若虫（13.1%）。成年雄蜱和雌蜱的感染率相似（分别为19.5%和20.7%）。使用基因组种特异性寡核苷酸探针通过反向线杂交（RLB）鉴定了狭义伯氏疏螺旋体、阿氏疏螺旋体、伽氏疏螺旋体和VS116组等基因组种。鉴定出的最常见的伯氏疏螺旋体基因组种是VS116（34.6%）、伽氏疏螺旋体（24.3%）和狭义伯氏疏螺旋体（18.4%）。阿氏疏螺旋体不常见（6.6%）。在13.2%的感染蜱中观察到多重感染。基因组种的分布显示出地理差异，并且似乎也受到栖息地性质的影响。广泛的基因组种似乎与栖息地中广泛的潜在储存宿主的存在以及伯氏疏螺旋体狭义种的高总体感染率有关。
