Alessio H M, Goldfarb A H, Cao G
PHS Department, Miami University, Oxford, OH 45056, USA.
Int J Sport Nutr. 1997 Mar;7(1):1-9. doi: 10.1123/ijsn.7.1.1.
Vitamin C (ascorbic acid) was supplemented (1 g/day) for 1 day and 2 weeks in the same subjects. Plasma thiobarbituric acid reacting substances (TBARS) and oxygen radical absorbance capacity (ORAC) before and after 30 min submaximal exercise were measured. Different vitamin C supplementations did not affect resting TBARS or ORAC. Following 30 min exercise, values for TBARS were 12.6 and 33% above rest with 1 day and 2 weeks of vitamin C supplementation, respectively, compared to 46% higher with placebo. ORAC did not significantly change (11%) after exercise with a placebo, nor when subjects were given vitamin C supplements for 1 day or 2 days (4.9% and 5.73%, respectively). TBARS:ORAC, a ratio representing oxidative stress, increased 32% (p < .05) with placebo compared to 5.8 and 25.8% with vitamin C supplements for 1 day and 2 weeks, respectively. It was concluded that exercise-induced oxidative stress was highest when subjects did not supplement with vitamin C compared to either 1 day or 2 weeks of vitamin C supplementation.
在同一组受试者中，补充维生素C（抗坏血酸）1克/天，分别持续1天和2周。测量了次最大运动30分钟前后血浆中硫代巴比妥酸反应物（TBARS）和氧自由基吸收能力（ORAC）。不同的维生素C补充方案对静息状态下的TBARS或ORAC没有影响。运动30分钟后，补充维生素C 1天和2周的受试者，其TBARS值分别比静息时高出12.6%和33%，而服用安慰剂的受试者则高出46%。服用安慰剂运动后，ORAC没有显著变化（11%），服用维生素C补充剂1天或2周的受试者运动后ORAC也没有显著变化（分别为4.9%和5.73%）。TBARS与ORAC的比值代表氧化应激，服用安慰剂时该比值增加了32%（p < 0.05），而补充维生素C 1天和2周时，该比值分别增加了5.8%和25.8%。研究得出结论，与补充维生素C 1天或2周相比，不补充维生素C的受试者运动诱导的氧化应激最高。
